🔥The 9th Round of Easy Loan, Earn $40 Reward is in progress❗️ ⏰ Promotion Period: January 15th - Feburary 15th, 2025 👉 Register now and check more details at gate.io/campaigns/358

🚨We are recruiting junior faculty🚨 This is a broad search in GI patho/physiology. But, pancreatic cancer researches, do take note! Please RT and share widely 🙏🏼😊

New paper where we integrate 3D histology (CODA) with IHC and imaging mass cytometry (IMC) to produce 3D single-cell immune maps in the vicinity of pancreatic precursor lesions (PanINs). Read about CODA+IHC/IMC = 3D immune maps here: biorxiv.org/cgi/content/sh…

3D analysis of PanINs & associated inflammatory milieu is fascinating, showing how the immune suppressive microenvironment in #PancreaticCancer is baked in really early on in microscopic precursor lesions. Preprint by Ashley Kiemen Denis Wirtz Laura DeLong Wood Ralph Hruban

I’m very excited to share this preprint! Using CODA to co-register serial H&E with IHC images, we map CD45+ cells around PanINs to identify striking heterogeneity in 3D and distinct immune hotspots and cold spots

From Luciane T Kagohara 🇧🇷🧬👩🏻‍🔬👩🏻‍💻 Ashley Kiemen Denis Wirtz & colleagues Johns Hopkins Kimmel Cancer Center PanIN and CAF transitions in pancreatic carcinogenesis revealed with spatial data integration sciencedirect.com/science/articl… Spatial transcriptomics of archival #PancreaticCancer precursor lesions, with validation

Taking a precancerous lesion for a spin. Via color coding, we show how the content in different tissue components (immune cells, fat, ducts, islets, blood vessels, etc) in the proximity of that pancreatic lesion. More on CODA+IHC here: biorxiv.org/content/10.110…

Nonlinear registration of hundreds of multiplex histological images to map inflammation in a large pancreas sample Done using CODA More here 3D mapping of immune cells near PanINs here: biorxiv.org/content/10.110…

I am happy to share our recently published paper on Cell Systems. In this study, we developed an integrated experimental and computational approach to profile PanINs and examine the surrounding microenvironment. sciencedirect.com/science/articl…

We are recruiting the Director of Clinical GI Pathology at Johns Hopkins Pathology. Apply to lead the clinical side of our wonderful division, with diverse & supportive colleagues, great cases & clinical partners, & stellar trainees, in lovely Charm City. Please share! pathology.jhu.edu/careers/gastro…

New preprint from Luciane T Kagohara 🇧🇷🧬👩🏻‍🔬👩🏻‍💻 @FertigLab Dr. Elizabeth Jaffee Won Jin Ho Ashley Kiemen Ashi_W & colleagues Johns Hopkins Kimmel Cancer Center Convergence Institute Spatial multi-omics reveal intratumoral humoral immunity niches associated with tertiary lymphoid structures in #PancreaticCancer immunotherapy

And Anirban Maitra was again faster to post! On an amazing teamwork led by Dimitri Sidiropoulos, we were able to profile PDAC tertiary lymphoid structures induced by immunotherapies. A quick thread below.

Not the official Science Advances cover of this month, but you can still find it in the current issue! Check our latest paper where we used 3D-CODA to design assembloids that mimic the complexity of human fallopian tubes. Paper:science.org/doi/10.1126/sc…

Thrilled to report Patho-DBiT, just published in Cell 😊. It allows us to directly “see” all kinds of RNA species on the same clinical FFPE tissue slide, including mRNA, miRNA, snRNA, snoRNA, tRNA, etc, and splicing isoforms, genetic alterations (SNV, CNV, etc). Really a fun,

Very excited to work with Rosie Sears @FertigLab Jonathan Brody PhD Ashley Kiemen and our whole team to understand pancreatic precancer progression in high risk individuals

Excited to share the speakers for the next HOPTEC webinar series (Oct 29th, 3pm PT time/10AM US time)! >Join us to learn more about the state-of-the-art research on alpha-particle radiotherapies, and tumor-targeting peptides with Prof. Sofou and Prof. Bernardes.

Our paper was recently featured: nature.com/articles/s4159… > We demonstrated how 3D-CODA imaging can be used to iteratively refine assembloid design by mapping their 3D tissue structures and comparing to in vivo tissue, ultimately improving the assembloids' structural accuracy.

Jobs at Johns Hopkins University jobs.jhu.edu/job/Baltimore-… We are actively seeking a new coordinator for our HPB surgery group. If you are interested, please apply.

Congratulations Johns Hopkins resident Eric Young- winner of the 2025 abstract award from the Pancreatobiliary Society at the 2025 USCAP meetings!

Excited to share our new paper, where we integrate 3D imaging and nuclear segmentation to profile cellular neighborhoods in human pancreas. We demonstrate spatial immune patterns and microenvironment heterogeneity missed in 2D. A scalable pipeline for high-res tissue analysis.

🤠 👉🏽 🗽 news: I will be joining NYU Langone Health on September 1, 2025 as Director of the GI Cancer Center & Associate Director of Translational Research at Perlmutter Cancer Center at NYU Langone Health. I am excited to start the next chapter of the Maitra Lab in NYC & build a strong GI cancer research program.