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Our work “A transcriptional switch governs fibroblast activation in heart disease” previously on bioRxiv is now online nature. A great team effort Gladstone Institutes Deepak Srivastava and all the fantastic collaborators. A new thread /1 👇 nature.com/articles/s4158…

Excited to share our new paper in Nature Chemical Biology on Precise genome editing across kingdoms of life using retron-derived DNA nature.com/articles/s4158…

I’m excited to introduce our Retro-Cascorder, out today in nature! This molecular device logs barcoded receipts of gene expression in a temporal genomic ledger. Sequence the ledger --> recover the history of gene expression. nature.com/articles/s4158…

Excited to share this piece by Maggie Chen at WIRED taking a close look at the Retro-Cascorder. Also great to see thoughts and comments from Harris Wang @TheresaLovele12 and Timothy Lu

Check out our new review/opinion on molecular recording of transcriptional events by Sierra Lear. We write about where the field is, where it might be going, and what's stopping us from being there now. sciencedirect.com/science/articl…

I had a lot of fun thinking, reading, and writing about how we can record transcriptional events in DNA, & I'm super thrilled to see this out now!

*Preprint Alert* This one’s in a new area for the lab: phage editing! We're using retrons to produce recombineering donors ~inside~ bacterial hosts. A wild-type phage infects the host and an edited phage comes out. biorxiv.org/content/10.110…

New Retro-Cascorder Protocol! We've been recording transcriptional event order in cells using retrons and CRISPR integrases. We've love to expand the users and developers of the technology. To make that a bit easier, we just published a Nature Protocols. nature.com/articles/s4159…

New preprint! Simultaneous multi-site editing of individual genomes using retron arrays. We're trying to make it easier to make multiple, non-adjacent, precise mutations to a genome without long cycles of editing, isolating , and editing again. biorxiv.org/content/10.110…

Thrilled to share the first piece of work from my postdoc! Great team effort to develop a new technology for multiplexed genome editing using retron arrays in both prokaryotes and eukaryotes! Check the thread below!

Now published in ACS Synthetic Biology! pubs.acs.org/doi/full/10.10… ACS Synthetic Bio Gladstone Institutes UCSF Bioengineering & Therapeutic Sciences

Continuous multiplexed phage genome editing using recombitrons go.nature.com/3yUNYTi

Multiplexed editing of phage genomes using retrons, now published at Nature Biotechnology with lots of new bells and whistles. chloe kate crawford Santi Bhattarai-Kline Darshini Poola Karen Zhang Alex González-Delgado Matías Rojas Montero nature.com/articles/s4158…

A new retron-based method from the innovative Seth Shipman lab now allows making multiple mutations in a phage genome in a single, easy process - from point mutations to small insertions and deletions Read the overview by Ilya Osterman nature.com/articles/s4158…

Hope we can still buy food coloring at the grocery store. I wanted to make a princess cake. White marzipan is NOT going to hit the same

Our lastest contribution. Did you know that current preoperative fasting policies have no evidence base? The vast majority of studies examining the relationship between different preoperative fasting policies and aspiration actually measured surrogate outcomes. These outcomes

New Preprint!! Alex González-Delgado accomplished a major feat on this one: ported retron recombineering, which we love so much in E. coli, into 14 new bacterial species via a massive collaborative effort involving 9 labs! biorxiv.org/content/10.110…

It’s kind of interesting, but in some ways AI is making CS much more like biology. We can observe and tweak what it does, but we really don’t know how it’s doing it. A science and engineering discipline rooted in empiricism over theory. I wonder if this trend continues.