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Why are there so few CHO cell models for studying protein aggregation? Lots of yeast ones and a few E. coli ones. Nonscalable ones include Zebrafish and C. elegans.

In reading Chang Liu's paper on using serine integrases to increase transformation efficiencies in the OrthoRep P1 plasmid, could not help but wonder. Why aren't there more library DNA transformations performed in yeast using integrases rather than homologous recombination?

Streptavidin beads, two years expired from Mitenyi Biotech. Should we still use for MACS?

Happy to share an authoritative, topical, and detailed review paper on cover all the latest efforts to engineer proteases, out now in Biotech Avdances. sciencedirect.com/science/articl… Led by Samantha Martinusen, with considerable efforts from many lab members.