🔥The 9th Round of Easy Loan, Earn $40 Reward is in progress❗️ ⏰ Promotion Period: January 15th - Feburary 15th, 2025 👉 Register now and check more details at gate.io/campaigns/358

Please join this plant gene network at plantgene.atlassian.net/wiki/spaces/PH… Look at all the experts who founded this network including my friend Wayne Parrott

You know CRISPR complexes can cut DNA/RNA….what if some of them cleaved proteins too? Today in bioRxiv, we (+ Omar Abudayyeh hnisimasu) continue our study of Cas7-11 and type III-E systems, revealing a new triggered protease function biorxiv.org/content/10.110… 1/6

Today we report in Nature Biotechnology the high-throughout phase-assisted continuous evolution of compact Cas9 variants that require only single-nucleotide pyrimidine PAM sequences (N4TN and N4CN). This work was a terrific collaboration with Mo Khalil. 1/8) drive.google.com/file/d/1qmF5G0…

BREAKING NEWS: The 2022 #NobelPrize in Physiology or Medicine has been awarded to Svante Pääbo “for his discoveries concerning the genomes of extinct hominins and human evolution.”

We are glad to share that India's GEAC has finally recommended the environmental release of GM mustard for developing new parental lines and hybrids. 1/3 BRIC-NIPGR Department of Biotechnology SERB Indian Council of Agricultural Research. SciCommIndia 🇮🇳 Manoj Prasad

Please retweet: Open postdoc position in our lab to characterize the role of plasmodesmata in plant development, focusing on cell to cell trafficking of the KN1/ STM class of homeodomain proteins and mRNAs. Experience in plant genetics and/or cell biology/ microscopy is desired.

Alyssa's leukemia went into remission by 1 month, and is clear 6 months later🤞. 9 more patients to follow. Much gratitude to Alyssa & her family, Waseem's team, Alexis Komor (Komor Lab) who led the development of CBE as a postdoc in our lab, & many others who paved this path.3/3

Ever noticed that #CRISPR HDR is great in some cells but terrible in others? Even editing the same site. Superstar postdoc Erman Karasu figured out why! TREX1 is a biomarker for HDR: TREX1-low = high HDR and TREX1-high = low HDR. Also designed an easy fix! biorxiv.org/content/10.110…

Wishing you Happy new year 🙏🏻🙏🏻

When supervisor becomes Santa 🎅 김재연 Jae-Yean Kim

Year end lab dinner 김재연 Jae-Yean Kim Rahul M Shelake Tien V. Vu Vu Huy Minh

The scientific and popular literature is full of claims for diets that delay or reverse the aging process (at least in model organisms). But how do these interventions work? A 2021 #ScienceReview explored the fact and fiction of antiaging diets. scim.ag/124

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks - MPIMP Potsdam Golm 🌱🧪 go.nature.com/3vDIiYd

"Precise cut-and-paste DNA insertion using engineered type V-K #CRISPR-associated transposases" Tou CJ, Orr B & Kleinstiver BP. (bioRxiv. 2022-01-09) Nat Biotechnol. 2023-01-02. doi.org/10.1038/s41587…

Programmable A-to-Y base editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase go.nature.com/3Cwgjh6

Delighted to share publication in mBio Dibyajyoti Pramanik, PhD 김재연 Jae-Yean Kim Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli | mBio journals.asm.org/doi/10.1128/mb…

Add a few nucleotides to the 5' end of a guide RNA: - Retain on-target editing. - Reduce off-target editing. Method to screen 10k-100k+ "extended" guide RNAs finds the best variants. 🔻 Another useful dataset for more precise gene editing models, too. biorxiv.org/content/10.110…

Thrilled to share this new paper from our lab. We show that Cas9 binds to hundreds of positions in bacterial genomes and can silence genes with as little as 4nt of complementarity between the guide RNA and the target. Nucleic Acids Res doi.org/10.1093/nar/gk…

Delighted to share the publication 김재연 Jae-Yean Kim Dibyajyoti Pramanik, PhD Plant Mutagenesis Tools for Precision Breeding: Conventional CRISPR/Cas9 Tools and Beyond link.springer.com/chapter/10.100…

Delighted to share a paper published in the BMB Reports Journal published by the KSBMB Society. 김재연 Jae-Yean Kim Dibyajyoti Pramanik, PhD “CRISPR base editor-based targeted random mutagenesis (BE-TRM) toolbox for directed evolution” bmbreports.org/journal/view.h…