RESTRICT-seq enables time-gated CRISPR screens and uncovers novel epigenetic dependencies of SCC resistance biorxiv.org/content/10.110… #biorxiv_cancer

🎉🎉🎉 #PrecisionCRISPRscreen

One of the biggest challenges in cancer therapy is tumor evasion of treatment. Unfortunately, there a few tools available to interrogate these dynamic processes with high precision. We engineered CRISPR-SPARK to solve this! Check out our latest study on #RESTRICTseq👇

We developed a LLM-based pipeline for multiplexed engineering of Cas9 proteins, allowing us to simulate and test various functional domain insertions that minimize disruptions to arCas9 allosteric functions. #RESTRICTseq #PrecisionCRISPRScreens #CRISPR

To optimize RESTRICT-seq without relying on extensive, time-consuming knockout assays for every condition, we developed the arCas9 Modeling for Activity Prediction (arMAP) algorithm. This revealed that nuclear localization of Cas9 serves as a direct proxy for its DNA cleavage.

Such a pleasure leading the junior faculty session at the 2025 Rising Stars in Engineering in Health event today! So amazed by the incredible talent currently entering the market !

Day 2 of Columbia BME Rising Stars in Engineering in Health was a huge success! So glad for the opportunity to reconnect with friends and role models!

Excited to have this up on pubmed now!