Congratulations Joe Muldoon (Josh Leonard and Bagheri Lab) on your thesis seminar today! #IBiSGrads #IBiSAlumni

Now on bioRxiv—we build upon and combine several mammalian syn bio technologies. Our approach enables the construction of a wide range of genetic programs, and it makes the leap from relying upon intuition to using accurate model-guided design. Very proud of the team.

A powerful approach for controlling delivery at the single cell level: this was a fun collaboration with HoracioEspinosa, Jack Kessler, and teams Joe Muldoon Northwestern CSB

Need a stocking stuffer? Our study on elucidating and refining synthetic receptor mechanisms is out in final version today Synthetic Biology! Northwestern CSB Northwestern Engineering doi.org/10.1093/synbio…

Looking to obtain COMET or mMoClo plasmids from Addgene ? We have deposited 291 plasmids and they are starting to become available on Josh Leonard Addgene Page. See thread below for more information and links:

Our new study on predictive design of mammalian genetic programs is out in Science Advances! We paired engineered genetic parts that have special capabilities with computational models to design circuits that perform sophisticated, useful functions advances.sciencemag.org/content/7/8/ea…

Nice piece on predictive design of mammalian genetic programs - a great collaboration with Bagheri Lab led by Joe Muldoon. Beautiful art by Justin Muir. Northwestern CSB NIBIB Science Advances advances.sciencemag.org/content/7/8/ea… news.northwestern.edu/stories/2021/0…

1/4 We’re excited to share two major papers out Science Translational Medicine showing the many benefits of synNotch CAR circuits for the treatment of solid tumors. Axel Hyrenius-Wittsten, an incredible postdoc Roybal Lab, led the studies in Ovarian Cancer and Mesothelioma. tinyurl.com/drje876u

1/4 Excited to share our new preprint from the Roybal Lab & Mo Khalil-Raymond Liu and Iowis Zhu led the development of NextGen synNotch receptors. SynNotch is one ‘species’ in a larger ‘genus’ of SyNthetic Intramembrane Proteolysis Receptors (SNIPRs) tinyurl.com/7kf6bdyr

Thanks NIBIB for highlighting this cool project led by Joe Muldoon with Bagheri Lab Northwestern CSB nibib.nih.gov/news-events/ne…

Very excited to be part of the Mnemo adventure and humbled to be a co-founder next to my former mentor Michel Sadelain, Isabelle Rivière and Sebastian Amigorena. endpts.com/michel-sadelai…

Excited to share new work w/ co-lead Camillia Azimi, @MarsonLab Jeff Bluestone Roybal Lab: using a method we call 'CAR Pooling', we tested many CARs in multiplex & found new non-T cell signaling domains which improve CAR-T efficacy. biorxiv.org/cgi/content/sh… 👇🧵 1/10

Are you interested in using mathematical modeling to help understand, refine, or employ your synthetic biology system, but don’t know where to start? Introducing GAMES—a model development workflow for rigorous characterization of synthetic genetic systems.

Check out GAMES - a model development workflow for rigorous characterization of synthetic genetic systems! pubs.acs.org/doi/10.1021/ac…

HLA-independent #Tcell receptors, in which the heavy and light chains of a CAR scFv are incorporated into the endogenous TCR locus, are more effective than #CD28-based #CARs at targeting #tumors with low antigen expression. #leukemia #AML @sloan_kettering nature.com/articles/s4159…

Today we report in Nature Medicine the HLA-independent TCR (HIT). Using CRISPR knockin, we remodeled the TCR specificity to target cell surface antigen in an HLA-Independent fashion and benefit from the TCR's unique sensitivity. 1/4 nature.com/articles/s4159…

Excited to share our paper Cell tinyurl.com/22s9pztn. Ray Liu and Iowis Zhu led the development of clinically optimized and tunable nextgen synNotch receptors dubbed SNIPRs for cell therapies. Thank you UC San Francisco Gladstone Institutes Parker Institute for Cancer Immunotherapy press: tinyurl.com/2p8usz7z

Thrilled to share the first Eyquem lab paper! Today we report in Cell evolving the AAV6 capsid to enable efficient engineering of murine T cells. An exciting collab with @asokanduke and led by the brilliants William Nyberg and Jon Ark cell.com/cell/fulltext/… 🧵1/11

I’m excited to share our report - out now in Nature Biomedical Engineering - of a new approach to CRISPR delivery that is poised to transform the way engineered T cell therapies are manufactured. Visit tinyurl.com/CRISPR-PERC for “how to” details. It’s threadin’ time! (1/11) nature.com/articles/s4155…

Thrilled to report in Nature Biomedical Engineering our new effort to improve the manufacturing and access of gene edited T cells. Awesome collab with the Ross Wilson and @MarsonLab. We developed a protein-based delivery for CRISPR systems in human primary cells. A 🧵 by Ross 👇🏼