Purifying eukaryotic proteins & their complexes at the scale & purity needed for structural, functional or mass spec characterization can be a pain! Our new protocol preprint bit.ly/3T5D96c describes how anti-GFP & ALFA tag nanobodies can make your life easier (1/6)

3/ Reference Based Motion Correction, similar to Bayesian Polishing in RELION, uses a 3D map to optimize per-particle motion and improve resolution. Multi-GPU optimized, with support for multiple 3D classes, and reuse of dose weights in Patch Motion: guide.cryosparc.com/processing-dat…

I am happy to announce that we've recently posted our new manuscript, titled: ‘Cell Cycle Regulation has Shaped Budding Yeast Replication Origin Structure and Function’ on BioRxiv (biorxiv.org/content/10.110…). We hope you enjoy! Tweetorial below:

Looking for a #postdoc or #PhD in eukaryotic #DNA replication and #CryoEM? We are recruiting! The positions are fully-funded by the Novo Nordisk Foundation and come with access to cutting-edge facilities and fantastic colleagues in #Copenhagen. See links below for more information.

Excited to unveil insights from my Ph.D. research on RomX—a key regulator of motility in Myxococcus xanthus. Revealing RomX's structure & intriguing parallels in proteins across eukaryotes & prokaryotes!

I am glad to be involved in this amazing work spearheaded by Sukanya Chakraborty .

We're thrilled to announce our new preprint on the structure of the human alternative clamp loader, CTF18, bound to PCNA. A big shoutout to our star PhD student Giusy Briola for spearheading this work! biorxiv.org/content/10.110… #DNAReplication

How are DNA-protein crosslinks like camptothecin-induced TOP1ccs resolved? Check it out in our freshly baked Nature Communications paper: nature.com/articles/s4146… Julien Duxin

Thrilled to share our latest paper on RTEL1! Huge shoutout to our first author Giuseppe Cortone. The work uncovers the structural and biochemical properties of RTEL1's C-terminal region, highlighting its crucial role in genome stability and DNA repair🧬 onlinelibrary.wiley.com/doi/full/10.10…

BREAKING NEWS The Royal Swedish Academy of Sciences has decided to award the 2024 #NobelPrize in Chemistry with one half to David Baker “for computational protein design” and the other half jointly to Demis Hassabis and John M. Jumper “for protein structure prediction.”

I‘m happy to share our work on laser flash melting to overcome preferred orientation in cryo-EM samples! 🥳❄️🔬 More infos on the underlying mechanism of particle redistribution and the use for preferred orientation issues in the 🧵 or here: biorxiv.org/content/10.110…

Our study on how the human MCM2-7 helicase is loaded onto replication origins 🧬 is now online nature! 🎉 Our findings reveal that unlike in yeast, human MCM loading does not require ORC6 and can occur through multiple pathways. Read more here: rdcu.be/d1F6S

Our article "MCM double hexamer loading visualized with human proteins" published in Nature today! ❄️🔬🧬🎉🥳 Great collaboration with John Diffley's lab. Congratulations to Florian Weissmann, Julia Greiwe and all other authors! rdcu.be/d1F7m

A novel cryo-EM affinity-grid approach utilizing monodispersed single-particle streptavidin on a lipid monolayer is introduced to improve particle absorption, thereby addressing challenges like preferential angular distribution rdcu.be/d1MNM eBIC Beamline STRUBIOxford

HISTORICAL MOMENT FOR INDIAN CHESS 🇮🇳 Gukesh D Is World Chess Champion 👑🥹

We are excited to share our latest publication in Nature! Led by our outstanding postdoc, Taha Shahid, & PhD student, Ammar Danazumi, our study reveals how the SV40 LTag helicase melts and translocates on DNA, driven by an entropy-switch mechanism. nature.com/articles/s4158…

We are excited to present the first #cryoEM structures of human GABA transporter 3 – GAT3 – bound to an in-house selective inhibitor, SR-THAP, to substrate GABA, and in the substrate-free state! bioRxiv biorxiv.org/content/10.110…

Preprint of SharmaG_omics Lab @IITHyderabad’s recent web server & API toolkit is online now. #HOToffthePress Read it here: researchsquare.com/article/rs-658… ---------------- Try this tool using this link and let us know your suggestions: project.iith.ac.in/sharmaglab/alp…

New online! Cell cycle regulation has shaped replication origins in budding yeast bit.ly/4erd1h3

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it! guide.cryosparc.com/processing-dat…