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For night 13 of #31NightsofHalloween #MicrosCreepy, here are some Naegleria gruberi cells— an amoeba on the left and a flagellate on the right that accidentally made 6 flagella instead of 2! Actin: ghostly purple/gray Tubulin: blue/green

For night 14 of #31NightsofHalloween #MicrosCreepy here is an amoeba with a mitotic spindle! 💜actin 🧡tubulin

For night 15 of #31nightsofhalloween #microscreepy, here is a ghostly amoeba crawling through a narrow channel. This imaging technique is called IRM, and it only shows the footprint of the cell in contact with the glass.

For night 17 of #31NightsofHalloween #MicrosCreepy, here are some Dictyostelium cells streaming

This microscopic image from HHMI's Beautiful Biology is of two cytoskeletal proteins along with DNA in the metaphase stage of cell division — by Andy Moore of HHMI | Janelia and Erika Holzbaur of Penn. ⬇️ 🔗 hhmi.news/3Nw0LPz

For night 19 of #31NightsOfHalloween #MicrosCreepy, here are some amoebae huddling together. The blinking you’re seeing is the contractile vacuole filling and emptying—this is how many pond-dwelling protists deal with fresh water rushing through the membrane due to osmosis.

For night 20 of #31nightsofhalloween #microscreepy, here’s a cell exploding! 💥

For night 21 of #31nightsofhalloween #microscreepy here are some amoebae that are minding their own business until a drug is added (color change) and they attack each other! Okay, okay, they’re not really attacking, but they do tend to clump and crawl around each other!

For night 22 of #31NightsOfHalloween #MicrosCreepy, Here is my all-time favorite cell. The perfect amoeba ghost! 👻

For night 23 of #31nightsofhalloween #MicrosCreepy, here’s a blebby amoeba! I overlaid several frames of a movie into the same image, so you can see the motion of the cell (inverted phase contrast) and its mitochondria (purple/pink/yellow).

For night 24 of #31NightsOfHalloween #MicrosCreepy here are some amoebae using a fungus as a jungle gym!

Thanks Leah Rosin (Lee-ah Rose-in) for hosting an awesome unconventional model systems symposium at NIH this week! Lepidopterans, tree hoppers, and sea stars, oh my! And it’s always nice to have a mini camp(m)ellone lab reunion (CampelloneLab Barbara Mellone)

For night 28 of #31NightsOfHalloween #MicrosCreepy, IT'S ALIVE! These are reanimated cell "ghosts," which are cells that have had their membranes/cytoplasm washed away, leaving the cytoskeleton behind. Adding ATP activates myosin motors, which causes contraction!

For night 29 of #31NightsofHalloween #MicrosCreepy, here is a clump of cells getting obliterated in an experiment gone wrong!

For night 30 of #31NightsofHalloween #MicrosCreepy, here are some amoebae squished under a jello-like pad. The flashes you see are contractile vacuole pumping events-- this organelle colllects and expels water (like bailing water out of a boat!)

Happy Halloween! For night 31 of #31NightsOfHalloween #Microscreepy, here is a grand finale video!

Glad when people engage with evolutionary biology, but this kind of visual strongly enforces the fallacy of treating evolution as a directed process. Each one of those ancestral nodes fanned out to give us thousands of successful lineages alive today AND countless dead ends too

Happy #microscopymonday! I made a new amoeba mosaic this weekend out of actin polymer-stained amoebae

We printed a copy for the lab! Want one for your lab? Download it here: katrinavelle.wixsite.com/science/printa…

Your Action Is Needed! Congress needs to hear from scientists. Call your representatives to oppose funding freezes and restrictions on research. Find contact info at govtrack.us and make your voice heard today! #SciencePolicy ascb.org/science-policy…