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機能未知遺伝子の網羅的解析 ファージには無数の機能未知遺伝子が存在する。今回RNAを標的とするdCas13を用いた新しい系(CRISPRi-ART法)を確立。ファージの系統を越えて幅広く機能。ファージのフィットネスに重要な90以上の未知の遺伝子を明らかに すごいです！ nature.com/articles/s4156…

A transcription factor from the cryptic Escherichia coli Rac prophage controls both phage and host operons - PMC pmc.ncbi.nlm.nih.gov/articles/PMC11…

Staphylococcus aureus ST764-SCCmecII high-risk clone in bloodstream infections revealed through national genomic surveillance integrating clinical data | Nature Communications nature.com/articles/s4146…

Harnessing Diversity Generating Retroelements for in vivo targeted hyper-mutagenesis biorxiv.org/content/10.110… #biorxiv_synbio

The sequel to our DRT2 story is here, and this one is once again full of surprises! This time around, the Sternberg Lab teamed up with the Wiedenheft Lab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: biorxiv.org/content/10.110…

高知大学の高橋さんMichiko Takahashiとの共同研究成果がiScience誌からオンライン公開されました ピロリ菌に感染するファージを利用して、異なる宿主に多段階感染させる実験を行い、その感染価やエピゲノムの変化を解析した成果です まだPre-proof版ですがぜひ御覧ください cell.com/iscience/fullt…

A minimal CRISPR polymerase produces decoy cyclic nucleotides to detect phage anti-defense proteins biorxiv.org/content/10.110… #biorxiv_micrbio

「タンパク質編集」の時代へ DNAの編集と似たように、標的タンパク質内の特定領域を置換する新規手法を確立。タンパク質組み換え配列を基盤としてカットアンドペースト方式で編集を行う。生細胞でも利用できるほか、非生物学的ポリマー(PEG)も挿入可能 いやはやすごい science.org/doi/10.1126/sc…

Great to see our Google DeepMind Google AI AI co-scientist innovations (multi-agent idea generation tournament) ship fast with Google Cloud Agentspace! Incredible shipping momentum at Google! 🚢 Agentspace -cloud.google.com/products/agent… AI co-scientist - research.google/blog/accelerat…

ゲノム編集ツールを改良する CRISPRの祖先型ともいわれるシステムに着目し、計算機上のスクリーニングと進化に基づいた設計により改良版のNovaIscBを開発。小型のためAAVに搭載できるほか、ほかの酵素と結合させてエピゲノム編集も nature.com/articles/s4158…

Latest BREX story from the lab - turns out there is a nuclease after all! Congrats to Jenny Readshaw and the whole team, including wonderful collabs Barry Stoddard, Brett Kaiser and Darren Smith. Nucleic Acids Res New England Biolabs Durham BioSciences academic.oup.com/nar/article/53…

Cool defense today! #Phage small RNAs.

Phage DNA modifications evade defense: Phage DNA modification adds arabinose to cytosines via hydroxy linkage(5ara-hC) w/potential double & triple arabinosylation. Arabinosylated phages are protected from DNA-targeting CRISPR-Cas & restriction modification cell.com/cell-host-micr…

A Novel Insertion Site for Group I Introns in tRNA Genes of Patescibacteria | bioRxiv biorxiv.org/content/10.110…

📢Preprint out! Excited to share my final work from the @Soreklab! We mined phage dark matter using structural features shared by anti-defense proteins (viral tools that help phages bypass bacterial immunity) to guide discovery. Found 3 new families targeting immune signaling!

Excited to share our new work—PhageMaP, a high-throughput platform for genome-wide knockout screening in diverse phages across multiple hosts. PhageMap reveals essential, non-essential and conditionally-essential genes, interactors with host anti-phage defenses & enables rational

遺伝子組換え技術黎明期、大腸菌で異種タンパク質発現するときに「ストップコドンでちゃんと翻訳が止まるようにというおまじない」でTAAを二回繰り返すTAATAAという習慣があった。実は大腸菌リボソームリサイクル因子がストップコドンの次のTを好むらしく、タンパク質発現効率が上がる、という。

Happy to share that the final chapter from my PhD is now published! We engineered a phage λ mutant to remove its lysogenic potential and deliver an all-in-one CRISPR-associated transposon (CAST) system for targeted gene insertion within E. coli. doi.org/10.1073/pnas.2…

大腸菌で組替タンパク質を発現させるときも培地にエタノールを少量加えるとシャペロンの発現を誘導して目的タンパク質の可溶性が増すことがある