Active site assembly of [NiFe]-hydrogenase scrutinized on the basis of purified maturation intermediates | ChemRxiv doi.org/10.26434/chemr…

We dissected the assembly mechanism of the NiFe(CN)2(CO) active site and isolated previously uncharacterized intermediates of the hydrogenase catalytic subunit.

Great collaborative project from Lenz_Group Stefan Frielingsdorf involving UniSysCat - Cluster of Excellence members as well as national and international collaborators C. Van Stappen

From our collection celebrating 10 years of ChemComm Emerging Investigators: 'A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H' 🔓 by Vincent Group Oxford, Caroline Emilie Paul and first author Holly Reeve. rsc.li/3QnQlkb

Reversible Glutamate Coordination to High-Valent Nickel Protects the Active Site of a [NiFe] Hydrogenase from Oxygen Freie Universität UniSysCat - Cluster of Excellence Lenz_Group TU Berlin #Glutamate #Nickel #Hydrogenase #Oxygen pubs.acs.org/doi/10.1021/ja…

Lenz, Zebger, and Horch investigate O2-inhibited states of the unique [NiFe]-hydrogenase HtSH (doi.org/10.1126/scienc… Science Magazine), find the nickel ion switching between Ni(III) and, wait for it, Ni(IV) 🤩 Cool spectroscopy, out now with J. Am. Chem. Soc. doi.org/10.1021/jacs.2…

Research Highlight: A team of #UniSysCat researchers deciphers how a hydrogenase can make itself more tolerant against oxygen - a step that is important in developing #hydrogen #energy strategies & for regenerating nucleotide cofactors. #catalysis J. Am. Chem. Soc. unisyscat.de/news-events/ne…

nature.com/articles/s4158… I´m glad it is finally out our work on the assembly of the [NiFe]-hydrogenase active site.Lenz_Group Stefan Frielingsdorf Name darf nicht leer sein C. Van Stappen Christian Limberg TU Berlin UniSysCat - Cluster of Excellence Humboldt-Universität zu Berlin DESY (Deutsch) SPring-8 MPI-CEC Nature Chemical Biology #hydrogen #energy

Check out our new review on the use of [NiFe]-hydrogenases like Huc in fuel cells. Lots of potential, but also many challenges ahead. With ⁦Chris Greening⁩ , ⁦Ashleigh Kropp⁩, and ⁦Vincent Group Oxford⁩. portlandpress.com/biochemsoctran…

In our new joint article doi.org/10.1039/D3SC03… in Chemical Science, we have modified and analyzed the unique [4Fe-3S] cluster of a true O2-tolerant [NiFe]-hydrogenase, revealing a refined picture of O2-tolerant H2/H+ cycling. Lenz_Group, Stefan Frielingsdorf, Armstrong Group Oxford

NEW in Chemical Science 🌟 Stepwise conversion of the Cys6[4Fe–3S] to a Cys4[4Fe–4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase by Ingo Zebger, Oliver Lenz, Patrick Scheerer et al. 🔗 doi.org/10.1039/D3SC03… Lenz_Group Stefan Frielingsdorf Armstrong Group Oxford

Our preprint is out! We provide the most comprehensive characterization of the hydrogenase maturase complex HypCD. Using transfer assays, Alphafold predictions, cross linking MS (and more) we show how the Fe(CN)2CO fragment is safely transferred to the apo hydrogenase using ATP!

Work brought to an end by Anna Kwiatkowski in collaboration with many UniSysCat - Cluster of Excellence groups. Lenz_Group Christian Limberg P01 DESY TU Berlin Chemistry Stefan Frielingsdorf Maria Andrea Mroginski

We had a fantastic JBIC session EuroBIC-17, great science and a diverse line-up of speakers. Thanks to all speakers and the audience! Springer Chemistry #EuroBIC17

Diese Woche im Fokus des OFFENEN WISSENSLABORS! Lest rein, wenn euch interessiert, wie Enzyme es schaffen, super #nachhaltig Wasserstoff zu produzieren. #Energie #Katalyse

Die #Energiewende herbeiführen müssen letztlich wir Menschen, alle zusammen in Zusammenarbeit. Die Forschung von #UniSysCat an Enzymen kann aber neue Möglichkeiten eröffnen: wir lernen von den natürlichen Katalysatoren wie sie effizient und ressourcenschonend #Energie umwandeln.

Diese Forschenden arbeiten an Enzymen, die für die #Energiewende interessant sind: den Hydrogenasen. Hydro-was? 😜 Lest das Interview und holt euch Antworten: berlin-university-alliance.de/wissenslabor/s… #Energie #Chemie Berlin University Alliance

A Strep-Tag Imprinted Polymer Platform for Heterogenous Bio(electro)catalysis (Ingo Zebger and co-workers) TU Berlin UniSysCat - Cluster of Excellence Armel Waffo Giorgio Caserta Stefan Frielingsdorf Jovan Dragelj Lenz_Group. #OpenAccess onlinelibrary.wiley.com/doi/10.1002/an…

ATP-Triggered Fe(CN)2CO Synthon Transfer from the Maturase HypCD to the Active Site of Apo-[NiFe]-Hydrogenase | Journal of the American Chemical Society TU Berlin #ATP #Maturase #HypCD #ApoNiFeHydrogenase pubs.acs.org/doi/10.1021/ja…

doi.org/10.1021/jacs.4…. Happy to share that our work on [NiFe]-hydrogenase maturation is finally out in J. Am. Chem. Soc.. Work carried out by Anna Kwiatkowski (@lenz_group) featuring UniSysCat - Cluster of Excellence members Stefan Frielingsdorf Christian Limberg Rappsilber laboratory Adam Belsom Maria Andrea Mroginski and P01 DESY 1/3