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Does anyone have a favorite method for RNA-FISH they would recommend? (with IF in cell culture)

Does anyone know of a good dataset for translation efficiency (polysome profiling, ribo-seq, etc.) in HEK cells or rodent neurons? I'm having a surprising about of trouble finding untreated, non-comparative, processed, deeply sequenced data. Any help appreciated!

Proud to have contributed to this story from @lleybova and others, now out in Developmental Cell! We show that hair follicle cell fates are radially patterned by Wnt and Shh gradients and that position and fate determine morphogenetic behavior. nam12.safelinks.protection.outlook.com/?url=https%3A%…

What drives cytoplasmic mRNA organization? We created unbiased, genome-wide maps of mesoscale RNA-RNA spatial proximity to find out - Biggest surprise was impact of encoded protein function. Fantastic work w/ Lindsay Becker Sofi Quinodoz David A Knowles (@davidaknowles.bsky.social) biorxiv.org/content/10.110…