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Finding your glute-a-mate has never been easier! #iGluSnFR3

redcross.org.ua/en/2022/02/don…

We wrote a blog post in 2018 about the latest glutamate sensor - but times have progressed and so must we. Abhi Aggarwal updated our post to feature the new newest sensor: iGluSnF from Kaspar Podgorski. hubs.la/Q017BC5b0

Folks, first paper of our lab is out!! We discovered that a couple of trans-synaptic proteins, LGI1 and ADAM23, are acutely translocated to the synaptic surface during firing! This controls their abundance at clefts, impacting locally glutamate release. biorxiv.org/content/10.110…

Our group is looking for a Research Associate interested in helping test new fluorescent indicators in vivo. Fun team. Relocation support to Seattle. Know anyone? alleninstitute.org/careers/#Divis…

iGluSnFR3 is published! Check it out⬇️ nature.com/articles/s4159… We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission. This is #iGluSnFR3 in action detecting minis w/ TTX. Kaspar Podgorski HHMI | Janelia Allen Institute

🎉 Thrilled to share 4 yrs of hard work: iAspSnFR preprint at tinyurl.com/38ve4vh9 🔬 GFP-based aspartate sensor, engineered via deep mutational scanning, tested in HEK293 cells. Highly specific for intracellular aspartate! #iAspSnFR #biosensor #aspartate

I am thrilled to share my first bio paper ever in Adam Cohen lab with super important contributions from @rhodamine110 lab! We asked the question: How do neurons compute? We focused on L2/3 neurons. Come for the science, stay for the videos! (1/17) x.com/biorxiv_neursc…

We Allen Institute for NeurDyn-Ophys are gearing up to launch a platform for in vivo testing of genetic NM/NT/Voltage indicators. We want your opinion on the most suitable AAV serotype to deliver sensors to be tested. Which serotype do you think would be the best fit?

Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields. This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate. Can you tell where the magnet is? Want some? It's on Addgene now! addgene.org/219957/