Very excited to work with PixelgenTech on its Molecular Pixelation: biorxiv.org/content/10.110… Oligo-tagged Abs are used to quantify proteins in #SingleCells but also describe their spatial distribution (polarity) and protein-protein co-localization. 1/4

Excited to share some of our thoughts on Systems Immunology - together with Anna James and Petter Brodin sciencedirect.com/science/articl…

Happy to share our new results by Aron Arzoomand: "Too much of a good thing might be bad" at Keystone Symposia #KSMicroImmune24. Inspired by great talks from Michael Fischbach silverman lab HandLab @LHooperLab (Sean Spencer) The Sonnenburg Lab, Noah Palm...Thanks for inviting me Jorge

Amazing to be at #IUIS2023 in beautiful Cape Town and share our recent results on the Immunomodulatory effects of Testosterone in humans. Incredible meeting thus far!

🚨New paper alert! Restrained memory CD8+ T cell responses favors viral persistence and elevated IgG responses in patients with severe #LongCOVID medrxiv.org/content/10.110… a thread 1/6.

2/6. To maximise our chances of understanding mechanisms of disease we enrolled the 121 most severe cases among >1000 patients screened.

3/6. A key observation in #longCOVID is elevated IgG responses to SARS-CoV-2 which normally wane off but imply persistent antigen (viruses) in these patients.

4/6. Plasma spike and other antigens is found in ~10% of patients, but minimal overlap across assays and no correlation to symptoms or immune parameters. Possibly because viral reservoirs are not leaking into blood. Elevated IgG responses is a more sensitive marker of #LongCovid

5/6. But why do some people not clear SARS-CoV-2 viruses? We find restrained, but not exhausted, CD8 T cell responses that inversely correlate (clonal expansion) with IgG responses providing a possible mechanism of disease...

6/6. This work builds on the work of others (ex Klein et al, Nature, 2023; Cervia-Hasler et al, Science, 2024; Yin et al, Nat. Imm, 2024; Swank et al, 2022 etc) and advances our understanding of #longCOVID. Genetic analyses are now ongoing to find the cause of restrained

Our paper "Integrating population and single-cell variations in vaccine responses identifies a naturally adjuvanted human immune setpoint" John Tsang @tsanglab.bsky.social is now online Immunity Link to open access pdf: authors.elsevier.com/c/1j0dm3qNrUxt…

Molecular Pixelation PixelgenTech is out! Nature Methods -rdcu.be/dHi87 Study I) abundance, II) spatial distribution & III) co-localization of hundreds of proteins in thousands of individual cells by sequencing. New dimensions in single cell biology 🙏KAW

Where do #TissueTregs come from? The previous standard model is the "seeding and specialisation" model, where #Tregs enter from tissues, turn on a dedicated transcriptional program per #tissue, and dwell indefinitely in that tissue as specialised cells. 2/21

New paper out nature - why are males more vulnerable to severe infections (ex acute #COVID), while females suffer more from autoimmunity (SLE, MS etc and also #LongCovid)? sex chromosomes or hormones? Here we studied adaptation of human immune systems to gender-affirming

Happy and honoured to have contributed to this major project!