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Started the experimental portion of my honors thesis today! We will be performing an experimental evolution using thousands of barcoded yeast to explore how they adapt to two drugs as well as combinations of the drugs. #KGSlabEvolves KerryGeilerSamerotte Kara Schmidlin ASU School of Life Sciences ASU SOLUR

Completed the first transfer of the experiment today and froze down time point zero! #KGSlabEvolves KerryGeilerSamerotte Kara Schmidlin

Completed second time point of the evolution today, yeast seem to be growing well! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte

Completed time point 3! Today's stocks also filled our first freezer box! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte

Time point 5 complete. Today was our quickest transfer so far, Kara Schmidlin and I are starting to get the hang of things! #KGSlabEvolves KerryGeilerSamerotte

Completed time point 7 today and finished preparing our first samples from time point 0 for sequencing! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte

Completed time point 10 today and filled our third full box of glycerol stocks! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte

Happy Thanksgiving everyone! Time point 14 completed today. Thankful for the opportunity to work on this project in an amazing lab and that it's going well so far! #KGSlabEvolves KerryGeilerSamerotte Kara Schmidlin

Time point 17 complete! We're over halfway through the evolution now and our cell counts have been pretty interesting, check out Kara Schmidlin's tweet from today for more info and some graphs! #KGSlabEvolves KerryGeilerSamerotte Biodesign Institute at Arizona State University

Made it through time point 20 today! Only about a week and a half of transfers to go! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte Biodesign Institute at Arizona State University

We completed time point 22 today and did our last weekend transfer! We can't believe there's only about a week left in our evolution now! #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte Biodesign Institute at Arizona State University

Last transfer of the evolution completed! After we collect our last time point on Friday we'll be moving onto analyzing barcode frequencies next year. #KGSlabEvolves Kara Schmidlin KerryGeilerSamerotte Biodesign Institute at Arizona State University

Today I pipetted! First time back in the lab after a long time and I really missed it. Kara Schmidlin and Sam Apodaca evolved yeast to resist different drugs are now conducting a big screen for cross resistance/sensitivity. More updates soon! #KGSlabEvolves

Sad to miss #PEQG22 Genetics Society of America, but happy that my lab’s undergraduates and graduate students have the opportunity to share their research! Please check out talk #5 by Sam Apodaca & posters 212W by Rachel Eder; 382T by Daphne Newell; 306V by Natalie Quan; 316V by Gaurav Bilolikar

Excited for a great day of talks & posters at #PEQG22! Sam Apodaca starts off w/an exploration of tradeoffs/contingency in evolution of radicicol & fluconazole resistance in S. cerevisiae using a large dataset of barcoded samples evolved in multiple conditions