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It’s wonderful to see so many successful alumni and current lab members driving the field forward and making an enormous impact. I am very proud of all of you!

Published in PNAS: "4Pi MINFLUX arrangement maximizes spatio-temporal localization precision of fluorescence emitter" by J.D. Rickert, M.O. Held, J. Engelhardt & S.W. Hell. Check it out (open access) at pnas.org/doi/epdf/10.10… Max Planck Institute for Medical Research #MINFLUX #Fluorescence #Microscopy

Chemistry contributions – check them out if you haven’t already! A.N. Butkevich in Org. Lett.: pubs.acs.org/doi/10.1021/ac… K. Uno et al. in Org. Chem. Front.: pubs.rsc.org/en/content/art… D. Kim et al in JACS: pubs.acs.org/doi/10.1021/ja… #Fluorescence #Superresolution #Microscopy

Chemistry from the Hell Labs: D. Kim et al. in Chem. Methods: …mistry-europe.onlinelibrary.wiley.com/doi/full/10.10… A. Aktalay et al. in Angew. Chem. Int. Ed.: onlinelibrary.wiley.com/doi/full/10.10… E.A. Savicheva et al. in ChemPhotoChem: …mistry-europe.onlinelibrary.wiley.com/doi/full/10.10… A. Aktalay et al. in ACS Cent. Sci.: pubs.acs.org/doi/10.1021/ac…

Optical elements… with R. Orange-Kedem, Y. Shechtman et al. in Light Sci. & Appl.: nature.com/articles/s4137… J. Engelhardt, B. Ellerhoff, C.-M. Gürth, S.J. Sahl & S.W. Hell in J. Microscopy: onlinelibrary.wiley.com/doi/10.1111/jm… M. Leutenegger & S.W. Hell in Sensors: mdpi.com/1424-8220/22/2…

“Photoactivatable Large Stokes Shift Fluorophores for Multicolor Nanoscopy”. pubs.acs.org/doi/10.1021/ja… by I. Likhotkin, R. Lincoln, M.L. Bossi, A.N. Butkevich & S.W. Hell in JACS (2023). #Fluorescence #Dyes #Microscopy #Superresolution #Nanoscopy

Recent papers: G. Knorr, M.L. Bossi, A.N. Butkevich, S.W. Hell in Organic Letters (2024) pubs.acs.org/doi/10.1021/ac… M. Remmel et al. (2024) in Small Methods onlinelibrary.wiley.com/doi/10.1002/sm… M. Remmel, L. Scheiderer, A.N. Butkevich, M.L. Bossi,S.W. Hell in Small (2023) onlinelibrary.wiley.com/doi/full/10.10…

Nanoscopy, without the OFF… Check out the preprint by T.A. Hensel, J.O. Wirth & S.W. Hell (2024) “Diffraction minima resolve point scatterers at tiny fractions (1/80) of the wavelength” biorxiv.org/content/10.110… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Optics #Imaging #Microscopy #Superresolution

#MINFLUX tracking experiments in two color channels reveal a novel mode of kinesin stepping. Check out the recent preprint by L. Scheiderer, J.O. Wirth, M. Tarnawski & S.W. Hell “Dual-color MINFLUX: Kinesin-1 takes Chassé-Inchworm steps” biorxiv.org/content/10.110… Max Planck Institute for Medical Research

Published in Nature Methods (2024): L. Scheiderer, H. von der Emde, M. Hesselink, M. Weber & S.W. Hell "MINSTED tracking of single biomolecules" nature.com/articles/s4159… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Optics #Imaging #Microscopy #Fluorescence #Superresolution#Nanoscopy #MINFLUX #MINSTED

Published in Communications Biology (2024): J.O. Wirth, E.-M. Schentarra, L. Scheiderer, V. Macarrón-Palacios, M. Tarnawski & S.W. Hell “Uncovering kinesin dynamics in neurites with MINFLUX” nature.com/articles/s4200… Max Planck Institute for Medical Research #Microscopy #Superresolution#kinesin #protein #MINFLUX

#MINFLUX directly tracks CRISPR/Cas9-tagged endogenous dynein with nm/ms precision in living primary neurons, including direction reversals occurring rapidly at the time scale of single steps. Recent preprint by J.M. Schleske et al. biorxiv.org/content/10.110…

#MINFLUX fluorescence nanoscopy in biological tissue New preprint by T. Moosmayer, K.A. Kiszka et al. including PSD95 relative to the spine morphology (example below), presynaptic VGlut clustering and AMPAR clustering at the post-synapse. Check it out at biorxiv.org/content/10.110…

New article in Nature Communications by J.-E. Bredfeldt, J. Oracz, K.A. Kiszka et al. "Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm". Open access at nature.com/articles/s4146… MPI for Multidisciplinary Sciences #Optics #Imaging #Microscopy #STED

PNAS Inaugural Article published: "MINFLUX reveals dynein stepping in live neurons" by J.M. Schleske et al. Open access - read it at pnas.org/doi/abs/10.107… Max Planck Institute for Medical Research MPI for Multidisciplinary Sciences PNASNews #Optics #Imaging #Microscopy #MINFLUX #Fluorescence

Published in Science Magazine: "Direct optical measurement of intramolecular distances with angstrom precision" (Sahl et al.) science.org/doi/10.1126/sc… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Optics #Imaging #Microscopy #MINFLUX #Fluorescence

#MINFLUX detects two arrangements of a protein subunit dimer by directly resolving fluorescent markers at and below FRET-range distances. MINFLUX #imaging has gone inTRA-molecular! Learn more at science.org/doi/10.1126/sc… and mpinat.mpg.de/4816514/pr_2419 @mpinat Max Planck Institute for Medical Research #Optics #

Published in PNAS: T. Moosmayer, K.A. Kiszka et al. (2024) "#MINFLUX fluorescence nanoscopy in biological tissue". Open access at pnas.org/doi/abs/10.107… including PSD95, presynaptic VGlut1 clustering and AMPAR clustering at the post-synapse MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research

Optical nanoscopy, but without the OFF...! Published in Nature Physics by T.A. Hensel, J.O. Wirth, O.L. Schwarz & S.W. Hell. Check it out with open access at nature.com/articles/s4156… MPI for Multidisciplinary Sciences Max Planck Institute for Medical Research #Imaging #Optics #Waves #Microscopy

"Caging-group-free photoactivatable fluorophores with far-red emission" for #MINFLUX, #STED, #PALM superresolution imaging. New preprint by R. Lincoln, A.N. Butkevich et al. Check it out at biorxiv.org/content/10.110… Max Planck Institute for Medical Research MPI for Multidisciplinary Sciences #Nanoscopy