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Woah cool! I'm going to try this O2/O3 mount for my snoutscope build at the UC San Francisco CALM

Gentle Rhodamines for Live-Cell Fluorescence Microscopy | ACS Central Science pubs.acs.org/doi/10.1021/ac…

Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a collimated ~1mm⌀ 532nm laser aligned into the back of Nikon 40x0.95 air objective. Radially symmetric/concentric interference rings indicate good alignment.

Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, Jenny Sachweh, Bernhard Hampoelz and others uncover that the surrounding cellular milieu determines NPC composition via the nuclear transport system itself. #teamtomo #NPC A 🧵

Following a poke from Jan Becker, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all: beryl.mrc-lmb.cam.ac.uk/rlgc_notebook/ Feedback always welcome. x.com/JamesDManton/s…

how I label my hex keys

PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.

Correction from Tanner Fadero! It's actually the The Center for Advanced Light Microscopy (CALM) UC San Francisco that is building the #Snouty microscope! They have a great team there with Nico Stuurman, Kari Herrington and @micaelalasser (Instagram). A great location!😀

We present long-term stabilization of an oblique plane microscope (OPM). By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift. The feedback signal comes from a laser, no fluorescence is expended. biorxiv.org/content/10.110…

Alright, I'm calling it. I'm over to the other place (rhymes with clue guy). Find me at my new profile: tanner dash fadero at bee ess kay why dot social dot com.

I'm not on Twitter anymore, but if you are, you should follow this account. Or come over to bsky and follow us there!