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Nicolas, your nysbc email account seems to over its quota

The purification of MHJF from 48 liters was a disaster. We believe we have severe aggregation problems.

Jose and Nathan have worked very hard, including Saturday and the Monday holiday but we will not be able to deliver protein today

We have to start all over again. I want to do a 2 liter purification trial with all our targets before we commit to an schedule

Jose and Nathan need some experience with each target before they can guarantee delivery

I sent you a crystallization table by email

Ralph and I will set up 2D crystallization together sometime next week. Will take MCMJR1 and will set up a few conditions

using dialysis cassettes and buttons in the traditional way. We will make the lipids together.

We will have MCMJR1 for tomorrow's experiment, will pick it up in the morning and arrive at NYSBC around 10 am

Ralph please make dialysis buffer: 20 mM sodium phosphate pH 7.0 50 mM NaCl 2 mM MgCl2 1 mM EDTA 20 mM sodium azide

Make one liter of it at 10X , that is: 200 mM sodium phosphate pH 7.0 500 mM NaCl 20 mM MgCl2 100 mM EDTA 200 microM sodium azide

it should be 200 microM sodium azide, not mM as in the first tweet

We will use DMPC and DOPC as lipids

We will need about twenty 100 mL beakers (250 mL ones will do). I'll bring some from my lab.

Sorry 10 mM EDTA

Be there in ten

Fingers crossed. Don't expect crystals but just aggregates and vesicles will be great

Saw the mcmjr1 trial. Staining is suboptimal. Controls were good. Perhaps some reconstitution in dmpc. Need to discuss what to try next.

We have 2.5 mgs of mxmjr1 ready for more trials

Group meeting today cancelled