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All crosslinkers out there in the world, please have a look at this: arxiv.org/abs/2007.00383 Major community effort, making the method more reliable and informative. Thanks to Alex Leitner and Juri Rappsilber for making this happen!

We are happy to share that with you 🥳Studying the impact of sequential digest with trypsin on the use of alternative proteases pubs.acs.org/doi/full/10.10…

The power of crosslinking mass spectrometry in hybrid methods!

You are interested in the "Anatomy of a crosslinker" go-ahead and check out our review in Current Opinion in Chemical Biology 😊sciencedirect.com/science/articl… Adam Belsom

AddThis | Home authors.elsevier.com/sd/article/S13… Anatomy of a crosslinker

Check out our new preprint on AAV capsid assembly! Great work by T.p.woerner @hecklab. He used native MS to show that these capsids are super heterogeneous. biorxiv.org/content/10.110…

Very excited to see this in print online!! Was a pleasure to work with Alexander Leitner and Rappsilber laboratory on these recommendations.

We are living in exciting times!

I asked Prof. Lee Cronin how he defines 'Life' when he says he's creating it from scratch. His answer started with planes, and ended with my mind blown open — youtu.be/6q5FGuJXSwA

Check out T.p.woerner's latest on single particle charge detection MS @hecklab! He did an in-depth analysis of the stability of single ions in the Orbitrap leading to some big improvements in ion sampling and resolution. biorxiv.org/content/10.110…

Very happy to share my secret project for the last 3 years: we were busy getting AI to run a nuclear fusion reactor. Spoiler alert: it works! Deep reinforcement learning is pretty great at working with sci-fi things where human intuitions tend to break down.

Understudied proteins: opportunities and challenges for functional proteomics | Nature Methods - the Understudied Protein Initiative! Yeah nature.com/articles/s4159…

An open invitation to the Understudied Proteins Initiative | Nature Biotechnology - Sign up now! nature.com/articles/s4158…

We and the RBO Lab - TU Berlin are excited to present AlphaLink nature.com/articles/s4158… a modified version of the AlphaFold2 protein structure prediction algorithm that can directly incorporate experimental residue-residue contact data from crosslinking MS (and potentially other techniques)!

In collaboration with the RBO Lab - TU Berlin, we are pleased to present AlphaLink2 - a modified version of AlphaFold-Multimer that is capable of directly incorporating experimental residue-residue contact data from crosslinking MS (and potentially other techniques as well). (1/7)

As a final note, sulfo-SDA crosslinks allow AlphaLink2 to position the viral protein within the Cullin4 complex's experimental density. (6/7)

#AlphaLink2 goes #ColabFold 😎 Sergey Ovchinnikov #AlphaFold  biorxiv.org/content/10.110… colab.research.google.com/github/Rappsil…

Light-Induced Orthogonal Fragmentation of Crosslinked Peptides Juri Rappsilber et al. Check it out 👉 go.acs.org/6jI The University of Edinburgh

The AlphaLink2 paper is now online! It’s joint work with RBO Lab - TU Berlin and Jörg Stülke. AlphaLink2 integrates crosslinking MS data into AlphaFold-Multimer. AlphaLink2 really shines for challenging targets like antigen-antibody complexes. doi.org/10.1038/s41467…