Molecular Pixelation, developed by Fredriksson and colleagues PixelgenTech, is an optics-free method that uses DNA-tagged antibodies for assessing cell surface protein location at single cell resolution. nature.com/articles/s4159…

How do chromatin modifications quantitatively impact gene expression? How do sequence variants or combinatorial marks influence relationships? We developed a modular #EpigenomeEditing toolkit to systematically test these Q, published today 🧵… nature.com/articles/s4158…

Mick W@tson ↙️ Richard Sever Prachee Avasthi If we start explicitly giving up on trying to do things that could be exciting, we will lose a generation of scientists under the shadow of blandness. /end

Spatial multi-omics at subcellular resolution via high-throughput in situ pairwise sequencing | Nature Biomedical Engineering nature.com/articles/s4155…

How do enhancers work over distances that sometimes exceed megabases? Excited to share our work led by Grace Bower where we uncover a unique sequence signature globally associated with long-range enhancer-promoter interactions in developing limb buds: biorxiv.org/content/10.110… 1/

Do you really need to spread plasmid libraries on mountains of plates to get uniform growth, or can you just dump them in a flask and call it a day? We make huge plasmid libraries in the de Boer Lab, so we tested whether culture method really matters. 1/ biorxiv.org/content/10.110…

Why is pooled oligo synthesis so expensive? What are the current bottlenecks to cheaper oligos?

A reminder of what Twitter used to be like nytimes.com/2024/05/26/nyr…

Meet MagLOV: an engineered protein that responds STRONGLY to magnetic fields. This is a fluorescence timelapse of MagLOV in E. coli. We're waving a (small) magnet under the plate. Can you tell where the magnet is? Want some? It's on Addgene now! addgene.org/219957/

Nice try

A lab member is trying to establish a timeline for enhancer studies covering discovery, identification, activity, & enhancer-gene connectivity. Are we missing something that we must add to the timeline? Gene Regulation Transcription Factors Chromatin Haiku #epigenetics #Chromatin

We present: 'Multispectral live-cell imaging with uncompromised spatiotemporal resolution'. biorxiv.org/content/10.110… 1/16

I don’t know what I was expecting

New achievement unlocked: survived my first overnight call!

I just wrote a review on transcriptional regulation and I want to talk about something that has been bothering me for 5 years, which ended up changing how I think about science in general. A brief thread: authors.elsevier.com/sd/article/S09…

“The question was, would lessons in a field far from their own make them better at the observational and diagnostic skills that lie at the core of ophthalmology? And the answer is that it did, substantially.” aaojournal.org/article/S0161-…

Congrats to my friend and colleague Guosong Hong for his stunning and original discovery, published today in Science, on clearing tissues *in living animals* with a common food dye! The dye is tartrazine, used in Doritos! science.org/doi/10.1126/sc…

Haven't been as productive academically lately due to being back in residency, but here's a GitHub-style heatmap of how times I've been paged each day.

Excited to share our latest work! We developed a new method for studying RNA localization via proximity labeling: OINC-seq! In contrast to other proximity methods, labels deposited on RNAs are read directly by sequencing without a need for biotinylation. biorxiv.org/cgi/content/sh…

Here's the thing. We have **NO IDEA** how to pick good graduate students. I served on admission committees for 10+ years, and chaired a few, and what I learned is that all the spreadsheets of grades and test scores and recommendations and essays and publications and interview