Knowing how to give a good talk is a crucial skill to have, but it's mostly not formally taught very well. Here's a 🧵on my strategy for how to prepare for the presentation, actually present it, and then answer the questions.

Excited to share our new preprint: CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions biorxiv.org/content/10.110… Project led by Kampmann Lab student Emmy Li, check out her tweetorial here:

We are happy to share our latest work on #ambientRNA in single-cell and single-nucleus RNA-seq experiments, out now in Genome Biology! (1/7) link.springer.com/article/10.118…

The Department of State visa bulletin for Aug, 2023 was issued today. There is a lot not to like. EB-1 for India has retrogressed by more than ten years. This is terrible for EB-1 eligible individuals from India, and terrible for US efforts to attract the best and the brightest

In 21 papers across Science, Science Advances, and Science Translational Medicine, researchers with the #BICCN consortium present an atlas of the human and nonhuman primate brain in unprecedented detail. The results uncover the genetic organization of the human brain. scim.ag/4ro

U.S. Consulate General Chennai I m a postdoc researcher UCLA Pathology David Geffen School of Medicine at UCLA. Travelled to India for family emergency, submitted docs for visa renewal at delhi on 19th oct. Only update on visa status is application received. My return flight is on Nov 2 midnight. Can you help?

Over a decade in the making. Congratulations to all the co-authors for their hard work. Forward genetic screen using a gene-breaking trap approach identifies a novel role of grin2bb-associated RNA transcript (grin2bbART) in zebrafish heart function frontiersin.org/articles/10.33…

This week in Science, Science Advances, and Science Translational Medicine, the #PsychENCODE Consortium lay out findings based on examining human brains at the single-cell level. Learn more: scim.ag/73Y #DecodingTheBrain

Researchers UCLA & Penn led by Luis de la Torre-Ubieta & Michael Gandal have uncovered over 200,000 new gene transcripts in the developing human brain. Their findings could enhance our understanding of brain maturation & genetic risks for neuropsychiatric disorders. ucla.in/3wDH97t

Our paper on splicing isoforms in developing human brain is finally out in Science Magazine! So grateful to Luis de la Torre-Ubieta and Michael Gandal for letting me be part of this amazing work. Congrats Ashok Patowary, PhD Pan Zhang Connor Jops! science.org/doi/10.1126/sc…

A NEW study has unveiled the most detailed view of the complex biological mechanisms underlying autism, showing the first link between genetic risk of the disorder to observed cellular and genetic activity across different layers of the brain. 1/

In a new Science study, researchers lead one of the most comprehensive investigations to date of splicing and resulting isoform diversity in the developing human brain—a process with strong links to neuropsychiatric disorders. scim.ag/76V #DecodingTheBrain

Long-read sequencing for 29 immune cell subsets (Transcriptomic Resource of Immune Cells using Long-read Sequencing, TRAILS) reveals disease-linked isoforms nature.com/articles/s4146…

#AcademicFrustration When someone asked you what your contribution was to the project that you led for 4 years, where you created the basic framework, standardized the majority of the processes, kept quality high, and validated every step

How to write a grant? 1. Write it for the reviewer, not you, the applicant. 2. Communicate in stories. 3. Make your story cohesive—leave no puzzling gaps. 4. Make your story resonate to keep the reviewer reading. 5. Accept chance and noise in peer-review. pnas.org/doi/epub/10.10…

Hard to capture long isoforms in single-cell RNA-seq. We have finally optimized an existing method to capture the majority of expressed isoforms, including longer ones. Oxford Nanopore #LongRead #SinlgeCell #Isoform

In general single cell long read RNAseq don't capture longer Isoforms; but not anymore; we can do capture longer Isoforms with single cell RNAseq also.

New preprint from our lab led by an amazing team, Celine Vuong and Alexis Weber, were we employ single-cell multi-omics in the developing brain to uncover mechanisms of Down syndrome. #brain #singlecell #multiomics #DownSyndrome biorxiv.org/content/10.110…