Thrilled to share that EVOLVEpro is now published Science Magazine Since our preprint, we now demonstrate low-N eningeering of both antibody and enzymes. We hope this model will broadly useful to the protein engineering field. science.org/doi/10.1126/sc…

We are excited to report the discovery of TIGRs, a widely-occurring RNA-guided system found in bacteria and their viruses. TIGRs consist of a peculiar repeat region which is transcribed into RNA and processed into multiple guide RNAs to direct TIGR-associated proteins to their

Interested in learning more about genome editing tool dev & their application to treat human diseases? 🧬 🏥 We're hiring graduating seniors who are seeking ~2 years of post-bac research experience. If you're interested in gaining experience in next gen medicines, please reach

The sequel to our DRT2 story is here, and this one is once again full of surprises! This time around, the Sternberg Lab teamed up with the Wiedenheft Lab to study how DRT9 reverse transcriptases provide antiviral immunity. Here’s what we found: biorxiv.org/content/10.110…

So excited to share our new paper out today in nature. We repurposed R2 retrotransposons to create STITCHR, a tool that can make diverse edits in mammalian genomes including scarless multi-kilobase scale insertions nature.com/articles/s4158…

Excited to share my PhD work… and more CRISPR-Cas9 PAM variants than anybody asked for

Genomes encode biological complexity, which is determined by combinations of DNA mutations across millions of bases In new Arc Institute work, we report the discovery and engineering of the first programmable DNA recombinases capable of megabase-scale human genome rearrangement

"Does it work in human cells?" is a question I've gotten a lot this past year. Excited to share our latest work on bridge recombinases! It's been a lot of fun to lead this team effort. If you are excited to hear more, join for my thesis defense tomorrow at 3pm PT. Links below:

Today in Science Magazine we report the development of a laboratory-evolved CRISPR-associated transposase (evoCAST) that supports therapeutically relevant levels of RNA-programmable gene insertion in human cells, a collaboration with Sternberg Lab. 1/13 drive.google.com/file/d/1I-UbCR…

New pre-print(s) from the Sternberg Lab in collaboration with Leifu Chang! We uncover an unprecedented molecular mechanism of CRISPR-Cas12f-like proteins, which drive RNA-guided transcription independently of canonical promoter motifs. Full story here: tinyurl.com/mr3fd493

Our new preprint is live! doi.org/10.1101/2025.0… In collaboration with Sternberg Lab, we determined the cryo-EM structure of a dCas12f-σE-RNAP complex from F. taeanensis, revealing an RNA-guided transcription mechanism that bypasses canonical promoter motifs.

Excited to share my third and final PhD project! We explored the biological consequences of RNA-guided flagellin regulation in bacteria. This was a real labor of love - spanning microbiology, microscopy, mouse gut colonization, and more. Co-led with the brilliant Egill Richard.

Excited to share that my PhD thesis work is out in Science Magazine today. We demonstrate robust rearrangement of the human genome using bridge recombinases, performing programmable insertions, excisions, and inversions at megabase-scale.

Was so fun to chat on stage with Sydney Ramirez, Tomer M. Yaron-Barir, and Katherine MacPhail at the #STATSummit!

thankful to STAT for highlighting some of our recent work and excited to be in Boston this week for the #STATSummit and #RTN2025

Genome maintenance by telomerase is a fundamental process in nearly all eukaryotes. But where does it come from? Today, we report the discovery of telomerase homologs in a family of antiviral RTs, revealing an unexpected evolutionary origin in bacteria. doi.org/10.1101/2025.1…

Meet the 2025 #STATWunderkinds! This year, as in past years, we’ve found inspiring stories and innovative research. All are blazing new trails as they attempt to answer some of the biggest questions in science and medicine. trib.al/sDuraWH

Inspiring talk by Ben Kleinstiver on (i) Cas9 variants with ⬆️ PAM flexibility & specificity, and (ii) circular ssDNA as non-toxic template to insert long DNA fragments. #EMBOCRISPRcas & big hugh to #Torontobluejays 😅

New pre-print 🧬✂️! We show that paired prime editing can efficiently generate large deletions (>1 Mb! 🤯) with high precision and at scale. We use this to perform the first pooled prime deletion screen across the human genome 👇short thread biorxiv.org/content/10.110…