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We visualized a transient catalytic chamber on the giant pyruvate dehydrogenase complex from native cell extracts!The motivation was extraordinary to study this target with an array of methods! Ioannis Skalidis hotmail.comshonis @hamdiphilum Francis O'Reilly Rappsilber laboratory cell.com/cell-reports/f…

We're hiring postdocs. Think deep homology meets proteomics. Discover ancient biochemical systems, invent new tech, or study the structure/function/evolution of proteins in embryonic development. Please RT! Email CV, interests, & references' contact info to [email protected]

(1/10) We combined #AlphaFold and #CryoEM to derive atomic models of MegaDalton protein community members from cell extracts! The efforts from the #KastritisLab together with Grzegorz Chojnowski were extraordinary to reveal their architectures - see thread below: cell.com/structure/full…

The protein organization of a red blood cell...fun and fantastic collaboration w/ Edward Marcotte David Taylor Kevin Drew Kim Vanuytsel Claire McWhite Eric Verbeke cell.com/cell-reports/f…

A Bayesian Framework for Cryo-EM Heterogeneity Analysis using Regularized Covariance Estimation biorxiv.org/cgi/content/sh… #bioRxiv

Self Fourier shell correlation: properties and application to cryo-ET biorxiv.org/cgi/content/sh… #bioRxiv