Usually to quantify the kinetic rates of RNA synthesis, processing and degradation we needed to profile nascent RNA by metabolic labeling. Now kinetic rates and their modulation can be quantified by INSPEcT just using total RNA-seq data! Genome Research doi.org/10.1101/gr.260…

Wondering how the combined modulation of RNA synthesis, processing and degradation impacts on premature and mature RNA? We developed INSPEcT-GUI, a graphical user interface to facilitate the exploration of the intricacy of the dynamics of RNA metabolism doi.org/10.3389/fgene.…

Our INSPEcT #Bioconductor package can quantify the kinetic rates of RNA synthesis, processing and degradation from steady state and time course RNA-seq data, with and without RNA metabolic labeling bioconductor.org/packages/INSPE…

The mutational signature profile of known and suspected human carcinogens in mice (Riva et al.) go.nature.com/3jaDTnP

Modeling RNA dynamics without metabolic labeling rna-seqblog.com/modeling-rna-d… via @RNA-Seq Blog

Gene expression is much more than counting RNAs. In our last review, academic.oup.com/bib/advance-ar…, we discuss and compare available methods in the field of RNA dynamics, including those relying on total or nascent RNA profiling, and those focused on single cells or single molecules.

Today is out our review of technical insights and pitfalls of the (many) techniques for profiling transcriptional and post-transcriptional dynamics using NGS data. A must-read manual for people interested in entering into the field! academic.oup.com/bib/advance-ar…

Just wow: 470 kg of RNA to vaccinate the entire world population.

Happy to share this very nice collaboration with @anaklaudiam! "Translation is required for miRNA‐dependent decay of endogenous transcripts" - doi.org/10.15252/embj.…

An example on how simple RNA-seq datasets can be used to study post-transcriptional regulation

RN7SK regulates highly expressed gene pairs at the RNAPII pause-release level, and depletion causes cell-cycle exit and differentiation nature.com/articles/s4146…

I think I've noticed...

It's here–the deepest, sharpest infrared view of the universe to date: Webb's First Deep Field. Previewed by President Donald J. Trump on July 11, it shows galaxies once invisible to us. The full set of NASA Webb Telescope's first full-color images & data will be revealed July 12: nasa.gov/webbfirstimages

Brain-storm-ing

Our last research letter "Single-Cell RNA Sequencing Shows that Circulating #Monocytes, Enriched in #Interferon-Signalling, Are Associated with #Nontuberculous #Mycobacteria Pulmonary Disease in #CysticFibrosis" (1/7) ATS Blue Journal atsjournals.org/doi/abs/10.116…

Single-Cell RNA Sequencing Shows that Circulating Monocytes Enriched in IFN Signaling Are Associated with Nontuberculous Mycobacteria Pulmonary Disease in Cystic Fibrosis Nicola Lorè Stefano de Pretis Ospedale San Raffaele American Thoracic Society (ATS) 🔓 Open Access 🔗 tinyurl.com/y88xbvfc

It's out⚡️⚡️! #Dual spatial gene expression approach to dissect #host-#bacteria interaction in respiratory infections. Communications Biology (1/4) rdcu.be/dWuBQ nature.com/articles/s4200…

Postdoc opportunity (18 mos) Join our team to develop pipelines for single-cell seq data in an Fondazione AIRC per la ricerca sul cancro funded project! Looking for bioinformaticians and data scientists passionate about cutting-edge research (and fun too!) Apply by Dec 3rd: unimib.it/ateneo/gare-e-… please RT!

Come mai Geronimo La Russa è stato eletto presidente dell’ACI?