If you liked the versatile TR-FRET methodology we reported in our cyclimid paper, check out our detailed STAR Protocols procedures. We are looking forward to seeing these approaches being (hopefully) broadly adapted! Ralph Mazitschek @drcmwoo & Saki Ichikawa sciencedirect.com/science/articl…

Thrilled to share our groups' new SEE-CITE photoaffinity labeling methodology where we installed a silyl cleavable linker between the diazirine and MOI to enable head-to-head interactomics. Spearheaded by Sho Takechi and Chau Ngo and now up on ChemRxiv chemrxiv.org/engage/chemrxi…

MIT researchers have developed a new technique using click chemistry to capture which genes are in the process of being transcribed in single cells, which they say could be the key to better understanding and treating some genetic diseases. cen.acs.org/biological-che…

Ten Simple Rules for Making Good Oral Presentations (2/2)

The 10 biggest cancer papers of the last 10 years (in my opinion) 1. Blocking PD1 activates the immune response against cancer - especially if the cancer has more mutations nejm.org/doi/pdf/10.105…

Here’s a reasonable roundup of the heterobifunctional degrader space for leukemia-associated targets. onlinelibrary.wiley.com/doi/10.1002/mc…

Science Magazine alphafold Pamela Ting Perspective: science.org/doi/10.1126/sc…

Big day for the lab as our MultiMap platform is now published on Science! Congratulations to Lindsey Zhi lindsey Lin and the team! science.org/doi/10.1126/sc…

Proximity-inducing #molecularglues for RAS- and RAF-dependent cancers

🚨New bioRxiv from the Amgen 🧪🔬🧬 Induced Proximity group. Here we introduce LYMTACs: Chimeric Small Molecules Repurpose Lysosomal Membrane Proteins for Target Protein Relocalization and Degradation. 🧵 biorxiv.org/content/10.110…

Here is something really cool!! Check out how IPP-Amgen colleagues Dhanusha Nalawansha Ryan Potts co-opted LMPs for targeted membrane protein relocalization and degradation by the lysosome. Single modality, multi pharmacology. #LYMTACs #TPD #PROTACs biorxiv.org/content/10.110…

Today we report in Nature Medicine the development of a base editing strategy for prion disease, currently a fatal and rapidly progressing neurogenerative condition with no effective treatment. @PrionAlliance DevermanLab (1/13) drive.google.com/file/d/1DVpKrz…

Covalent targeting of splicing in T cells by Kevin A. Scott, Hiroyuki Kojima, Nathalie Ropek, Ekaterina V. Vinogradova Katya Vinogradova and colleagues at Rockefeller University TPCB Weill Cornell Medicine Memorial Sloan Kettering Cancer Center Scripps Research dlvr.it/THQfxm

Cells are filled with toxic stuff that damages healthy proteins. Is that garbage just left to rot on the curb? No way! Ubiquitin ligases have evolved to recognize chemical damage and clean it up! nature.com/articles/s4158…

Tetanus on demand! (not for bioweapons, but for neuroscience research) Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release. To harness this toxicity and use it as a beneficial tool for

📢Excited to introduce NanoCas -our new mini CRISPR system that can reach tissues previously out of reach! By shrinking CRISPR to 1/3 its normal size, we can now edit genes in muscle, heart & brain that were difficult to access before. Summary & link to paper:

A pair of nature papers finally describe the structure of apoB-100 (the key protein of LDL) and how it interfaces with LDLR using new structural techniques along with AlphaFold2. nature.com/articles/s4158… nature.com/articles/s4158… (coverage nature.com/articles/d4158… Nature News & Views)

PROTACs coupled with oligonucleotides to tackle the undruggable tandfonline.com/doi/full/10.10…

Today in nature we share our back-to-back stories with Ning Zheng revealing chemical-genetic convergence between a molecular glue & E3 ligase cancer mutations. 1/6

I was happy to write a nature News and Views on the amazing discoveries by Brian Liau & Ning Zheng lab on how a molecular glue unexpectedly mimics the effect of cancer mutations. Such cool science and with implications to drug discovery. Nature News & Views nature.com/articles/d4158…