How are #ribosomes assembled 🏗️ in human cells?   In our new paper out in Science Magazine, we used genome editing 🧬 and #cryoEM 🔬❄️ to reveal the molecular details of how the large ribosomal subunit (60S) is assembled in human cells. (1/n)   👉 science.org/doi/10.1126/sc…

We are recruiting an engineer to work at our facility at french national #cryoem center neer Strasbourg, France. Our center belongs to the Instruct-ERIC and FRISBI Infrastructures. emploi.cnrs.fr/Offres/CDD/UMR…

Congrats to Patrick Schultz's team for their article on the structure of the NuA4/Tip60 complex. They reveal its structure, how and why it acts at a distance from its binding site. Great collab with Dom Helmlinger CRBM 📕 Nature SMB 📰 tinyurl.com/35uft9ye

Bored to look at schematic representation of #streptavidin affinity grids ? Here is a real image of the 2D crystal folding on itself. One can see within an unique micrograph both frond and side views of the affinity grid capturing its prey through biotinylated DNA !

Our latest, parallelized in-house #Python script for periodic signal subtraction for #streptavidin affinity grids, inspired from the work of Schultz and co-worker (doi:10.1016/j.jsb.2004.02.001), can process 6400 micgrographs in ~45 minutes with 16 CPU !

Happy to share our latest work on DNA topology regulation ! With cryo-EM and magnetic tweezers, we unveil how DNA gyrase captures a DNA crossover in supercoiled minicircles. doi.org/10.1126/scienc… Inserm CNRS 🌍 IBENS Université Paris Cité BCMHouston From the Labs at Baylor College of Medicine IGBMC

Happy to share the work carried out by Valerie Lamour lab IGBMC In collaboration with our #cryoem facility BioS-ME and Université Paris Cité BCMHouston. A major publication involving our #streptavidin grids ! doi.org/10.1126/scienc…

We are thrilled to share the work in which our facility took part, published in Science Magazine this week ! Valerie Lamour lab IGBMC, and collaborators IBENS From the Labs at Baylor College of Medicine solved the first structure of E.coli DNA gyrase recognizing a DNA cross-over. science.org/doi/10.1126/sc…

New publication! We succeeded in crystalizing a designed imaging scaffold (based on a protein cage), opening the possibility to use them to determine the structure of cargoes and small proteins in X-ray crystallography in addition to cryo-EM! Link: doi.org/10.1107/S20532…

Ce weekend, sur l'aérodrome historique du polygone Strasbourg.eu, venez rencontrer les associations aéronautiques présentes sur le terrain pour nos portes ouvertes. Possibilité d'activités aériennes sur place. https://://atap-polygone.fr/2024/09/14/portes-ouvertes-2024/

Excited to share a pre-print by Vita Vidmar and Céline Borde in collaboration with olivier espeli and Valerie Lamour about the interplay of #Transcription and DNA topology: biorxiv.org/content/10.110…

Bon…. Voilà ti pas que la lithothérapie pénètre au lycée. Le progrès fait donc rage. J’en déduis que ce n’est plus la peine de se fatiguer à expliquer, de s’échiner à vulgariser, de s’époumonner à transmette. La bataille est perdue. Reste, peut-ètre, l’héroïsme.

We are excited to share our latest work on the cooperation between RNA polymerase and the ribosome in bacteria: science.org/doi/10.1126/sc… The work was supported by IGBMC, Inserm, CNRS 🌍, -, @ERC_research, IMCBio Graduate School, and Instruct-ERIC. 1/7

Et on leur souhaite de le faire voler longtemps. C'est un bon avion. On le sait.

🇺🇦

United-States of shAmerica U.S. Embassy France

That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv! biorxiv.org/content/10.110…