[The Novice Scientist - Edición Nº 27-28] ¡Este sábado 12 de febrero a las 5:00pm ⏰, estará disponible la 27-28va edición de #TheNoviceScientist! ¡No te lo puedes perder! 🤩😎 #Divulgacióncientífica #Thenovicescientist

Nuestro laboratorio en UPCH abre convocatoria para pasantías en mayo-julio 2022. Buscamos estudiantes de 4to y 5to año de todas las universidades y carreras que quieran desarrollar su tesis en el lab. Pueden postular llenando el formulario: forms.gle/moCRS2QCYdigLF… ¡Gracias!

I’m excited to introduce our Retro-Cascorder, out today in nature! This molecular device logs barcoded receipts of gene expression in a temporal genomic ledger. Sequence the ledger --> recover the history of gene expression. nature.com/articles/s4158…

Are you an expert in Gene Editing🧬, WasteWater Management 🚰or related fields? Wageningen University students researching for the UN invite you to fill out a 5-10 min survey to help investigate frontier techs for sustainable development. Please share! forms.gle/x65iZfE7x6qBcm…

*Preprint Alert* This one’s in a new area for the lab: phage editing! We're using retrons to produce recombineering donors ~inside~ bacterial hosts. A wild-type phage infects the host and an edited phage comes out. biorxiv.org/content/10.110…

New Retro-Cascorder Protocol! We've been recording transcriptional event order in cells using retrons and CRISPR integrases. We've love to expand the users and developers of the technology. To make that a bit easier, we just published a Nature Protocols. nature.com/articles/s4159…

New preprint! Simultaneous multi-site editing of individual genomes using retron arrays. We're trying to make it easier to make multiple, non-adjacent, precise mutations to a genome without long cycles of editing, isolating , and editing again. biorxiv.org/content/10.110…

New Preprint, New Retrons! More than a hundred of them. We synthesized a ton of bioinformatically predicted retrons and put them through a battery of tests, from RT-DNA production to editing in human cells, E. coli, and phage biorxiv.org/content/10.110… Gladstone Institutes UC San Francisco UCSF Bioengineering & Therapeutic Sciences

Forget Hawaii, Mallorca or the Maldives, this is microbiologists' favorite vacation destination maps.app.goo.gl/GuD8LhGHdwk4b2…

I am delighted to share the newest work from the Sternberg Lab, in which we discover RNA-templated gene creation as a mechanism of antiviral immunity. This is a story for anyone who loves molecular biology, and one that repeatedly left us in disbelief. doi.org/10.1101/2024.0…

📢 Deadline EXTENDED! Apps due MONDAY to be part of GSMI as a grad school applicant or mentor!! ⁠ Sign up to participate or be a mentor by 6/10: cientificolatino.com/gsmi MORE INFO in 🧵 below x.com/cientificolati…

Multitrons: simultaneous multi-site editing of individual genomes using retron arrays, now published in Nature Chemical Biology Nature Chemical Biology: nature.com/articles/s4158…. Great job Alex González-Delgado and oso !

So excited to share one of the final works from my PhD in Seth Shipman’s lab with Asim Khan and oso, where we used high-throughput libraries and machine learning to create design rules for retron gene editors: biorxiv.org/content/10.110…

My favourite discovery ever has just come online. Can I please tell you about some seriously wacky molecular biology? The story starts with a reverse transcriptase that SOMEHOW defends bacteria from viruses. (👇 I recommend sound ON for the video 🎹) 1/

Multiplexed editing of phage genomes using retrons, now published at Nature Biotechnology with lots of new bells and whistles. chloe kate crawford Santi Bhattarai-Kline Darshini Poola Karen Zhang Alex González-Delgado Matías Rojas Montero nature.com/articles/s4158…

Very glad to have contributed to this project to broaden our knowledge on the very cool field of retrons and its applications. Especially in the company of such amazing scientists at the Shipman Lab! 🙌 Feeling very lucky.

We just posted a new preprint where we found retrons in bacteria out in the real world, in dirt and water. We describe the first retrons in a handful of new species, show how they defend against phages, and use them to edit genomes. Read about it here: biorxiv.org/content/10.110…

The June issue is live nature.com/nbt/volumes/43… On the cover, Khan et al. present a molecular census of retrons, performing the same battery of tests across many distinct systems to identify similarities and differences go.nature.com/3MOsMBx

New Preprint!! Alex González-Delgado accomplished a major feat on this one: ported retron recombineering, which we love so much in E. coli, into 14 new bacterial species via a massive collaborative effort involving 9 labs! biorxiv.org/content/10.110…