🔥The 9th Round of Easy Loan, Earn $40 Reward is in progress❗️ ⏰ Promotion Period: January 15th - Feburary 15th, 2025 👉 Register now and check more details at gate.io/campaigns/358

We're hiring! My lab at Berkeley is looking for a lab tech to join in our work on mRNA design. Are you excited about massively parallel reporter assays and RNA sequencing? Do you like bringing order to (minor) chaos? Apply here: careers.insidehighered.com/job/2386301/st…

What if proximity labeling enzymes (e.g., TurboID) can be activated by Ca2+ transients, just like GCaMPs? We developed Cal-ID, a Ca2+-dependent proximity labeling enzyme. It seems to label active neurons well, here is our preprint: biorxiv.org/content/10.110…

Berkeley students: my fall class on functional genomics is finally in the catalog in time for this week's enrollment period. You can find it as bioeng 190 and 290 and cmpbio 290. It's less math-intensive than my machine learning class and open to a wider range of backgrounds.

Hi all, we have a job opening for a Staff Reseach Associate (SRA) in our neurobiology lab at UC Berkeley. Looking for someone to help with lab manager duties, genotyping, etc. Check out job ID 38690 on careers.berkeley.edu. Please spread the word! UC Berkeley MCB Berkeley Neuroscience

🎆Preprint alert from Connor Horton and team!🎆 tinyurl.com/STRpreprint Short tandem repeats (STRs) are enriched in eukaryotic regulatory elements from yeast to humans AND polymorphic STRs change gene expression -- but why?? 🤔@StanfordChEMH @CZBiohub @StanfordBioE (1/8)

Psix, our new method for finding alternative splicing changes in single-cell RNA-seq data, is now out in Genome Research! Fantastic work from Carlos Buen Abad Najar, working with me and Nir Yosef, with contributions from Prakruthi Burra. 1/n genome.org/cgi/doi/10.110…

Hey Bay Area RNA enthusiasts! Super excited to announce the 2022 Bay Area RNA Club BARC meeting! #SaveTheDate for December 15 for a conference about all things RNA. In person, at UCSF, free. #BARC2022 #RNA

Our new tool for correcting sequence bias in ribosome profiling data, choros, is now live on biorxiv and GitHub! Fantastic work by Amanda Mok who recently finished her PhD in my group. biorxiv.org/content/10.110…

New paper alert! We (coincidentally) found a fungus resistant to translation inhibitor rocaglates and how it can escape from the toxicity. Congrats, Mingming, Kumakura-san, and the team! doi.org/10.7554/eLife.…

New work from Ron Kopito and Francesco Scavone reveal that UFMylation of ER translocon-bound 60S ribosome subunits promotes proteasome-mediated degradation of arrest polypeptides following ribosome stalling. 👏 biorxiv.org/content/10.110…

Congrats to MCB's Marla Feller Dr. Marla Feller and Donald Rio for being elected to the National Academy of Sciences National Academy of Sciences!! 👏🥂 bit.ly/3VFxDsB

I'm excited to share that my main project with the Ingolia Lab is finally published online! This culminates years of our hard work - I hope it fuels more exciting breakthroughs in the study of post-transcriptional regulation! nature.com/articles/s4159… Nicholas Ingolia

Ingolia lab carried out a systematic survey of the budding yeast proteome and identified hundreds of post-transcriptional regulators. Regulatory activity often lies within intrinsically disordered regions Nicholas Ingolia Kendra Reynaud nature.com/articles/s4159…

Berkeley students! If you want a hands-on introduction to single cell RNA-seq, epigenomics, and more, register for my fall class, Computational Functional Genomics! Undergrads: BioE 190, classes.berkeley.edu/content/2023-f… Grads: same, but 290. Email me for syllabus and prereqs.

Yeast RNA cross linking question. Does anyone know of a lab that has used psoralen TEG azide successfully in yeast? This is a the psoralen from the COMRADES method that allows for biotin to be added after RNA purification using click chemistry. Thanks! #yeast #rna

Check out this work from our lab on genome-wide single-gene CRISPR/Cas9 knockout screens to comprehensively interrogate CFTR- F508del ERAD. biorxiv.org/content/10.110…

Excited to share the final form of this excellent work by Lucas Ferguson: streamlined ribosome profiling, in collaboration with Kathy Collins to use her OTTR approach for library generation, and many other improvements

Cal-ID, a calcium-dependent protein proximity labeling tool, is finally published Nature Chemical Biology! We (Nicholas Ingolia) present a simple, scalable, in vivo compatible, and versatile platform that connects calcium signals to biochemical labeling of proteins. rdcu.be/dFDrb

We use Ca2+ indicators as a proxy for activity, but have you wondered what molecular events occur within cells during activity? I’m thrilled to have collaborated with Wren Kim & Nicholas Ingolia on Cal-ID, a calcium-dependent TurboID, that can allow us to answer such questions.