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Just went for a walk to try to wake up. It did not help.

Reading grants that are in a completely different field from my own is hard. Like... I don't know what these things mean.

I have been awake for 5 hours and accomplished 0 work. I think I'm doing this wrong.

Dear NSF: Why do you make us include 'pending and other support' pages even if they are empty?

I am the worst reviewer ever. People really shouldn't ask me to be an expert on ANYTHING.

Listening to other people talk about all of the time of they are going to take off.

Little Man has his driving learner's permit. I'm old & terrified.

There seems to be some confusion in the world. I am NOT a bioinformatician.

Is it best to build a transcriptome assembly with only 1 representative from each of your treatment groups or build an assembly from all of your samples? We've done the former but I'm not sure if currently assemblers work better now for the latter. Matt MacManes

Someone just asked if I was interested in an Illumina NovaSeq.😂😂🤣🤣🤣😂😂😂😂🤣🤣 I can barely afford the service contract of my MiSeq.

Honestly, the increasing pressure to publish is making my papers worse, & I don't even have the time to care.

So, it seems like the $2 million grant I'm a co-PI on was awarded. Still waiting on some paperwork but 'yay'.

But now I actually have to write my own.

If I have transcriptome data (20 individuals with 60 million reads per individual), do I need a reference genome to be able to identify transposon-derived transcripts?

This tweet is me desperately wanting to work on transposons, stress, and invasive species but having 0 idea what I'm doing.

So far two of the studies that I did for my PhD have been confirmed with follow up studies that include a lot more genomic data. I'm so relieved.

*knocks on wood* It seems like today might be a good science day.

Trying not to have a panic attack with all of the things going wrong in the lab at the moment. Is it like this for every lab? Or am I just really sucking at the moment.