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Got a question for SciTwitter: does anyone doing high-throughput human cell lysate and NGS library workflow experiments with liquid handlers have advice on your preferred instrument, for these two makes/models: - Beckman Coulter Biomek FXP - Tecan Freedom EVO 150 Thanks!

Big update: we're hiring a new lab manager!! Come join a fantastic group of scientists in the Sternberg Lab at Columbia University, in the heart of New York City, and contribute to cutting-edge CRISPR and genome engineering research. Please re-post and DM me with your interest

Exciting new work from the lab, on the marriage of transposons, CRISPR-like nucleases, guide RNAs, and self-splicing ribozymes! Check out the thread below from (Twitter-less) lead author, Rimante. A wonderful team effort biorxiv.org/content/10.110…

Our latest work exploring ultra rare CRISPR systems with new big data algorithms has been published by Science Magazine! Feng Zhang Soumya Kannan. This thread will unwrap how we found needles in a haystack of metagenomes. Let's dive in! (1/8) doi.org/10.1126/scienc…

Ever wonder if nature already 'invented' RNA-guided transcription factors like CRISPRi/a? Then look no further... we found many examples of domesticated, nuclease-dead TnpBs that use guide RNAs to repress genes! In bacteria AND phage! A wonderful team effort led by Tanner Wiegand

Excited to see this out -- congrats! (Now I have a link to share, as I keep telling colleagues to think (more) carefully about reaction buffer conditions! :)

Our latest research, published in PNASNews, identifies nearly 1 million OMEGA-TnpB & CRISPR-Cas12 genome editors. Key insights? RNA-guided activity is linked to more processes than we thought: transcription, transposition, motility and more! doi.org/10.1073/pnas.2… #CRISPR (1/3)

A tour de force of genome engineering and molecular detective work on one of my favorite mobile elements: integrons! Congrats Egill et al. - beautiful work 👌. Looking forward to the next one

Check out Diego's 🧵and excellent protocol on how to best harness CRISPR-associated transposases (CASTs) for bacterial genome engineering. We hope it will be a great resource for the community!

There were an insane number of "big" biotechnology papers published this week. The Bridge RNA gene-editing papers are important. But here are 7 more advances. Many new CRISPR tools, epigenetic editing for prion disease, gene drives for plants & more...🧵

We're excited to see our recent work on multi-functional transposons now in press. Nucleases and guide RNAs and catalytic introns, oh my! See the links and original thread below, for more... science.org/doi/10.1126/sc… drive.google.com/file/d/1fOVqT2…

Really proud to see this work published -- a thrilling detective story I was lucky to be a part of. Big kudos to Stephen Tang and the entire team for an impressive collaborative effort. See Stephen's thread below to learn more... (and read the paper!). drive.google.com/file/d/1_paZuA…