The fraction of cells in each phase should be proportional to the relative duration of the phase. We applied this model to cells growing at different growth rates, and .... molbiolcell.org/doi/10.1091/mb… pnas.org/doi/full/10.10…

To enable creative & influential research, promote good mentoring and teaching. The more innovative and provocative the research, the more it benefits from fresh perspectives. Teaching is a powerful multiplier of new thinking & approaches. nikolai.slavovlab.net/Research,-ment…

The epitranscriptome formed by the growing number of modifications occurring within mRNA transcripts.

When we started this project - about 10 years ago - we had limited access to old equipment. Better equipment would have been helpful, but using older equipment changed the perception about the feasibility & accessibility of single-cell proteomics. genomebiology.biomedcentral.com/articles/10.11…

Omics data cannot be better than the analyzed samples. This addresses the most frequent questions that I get for single-cell proteomic analysis of primary tissues.

𝐓𝐨𝐝𝐚𝐲 𝐦𝐚𝐫𝐤𝐬 𝐨𝐮𝐫 𝟏𝟎𝐭𝐡 𝐚𝐧𝐧𝐢𝐯𝐞𝐫𝐬𝐚𝐫𝐲 🎉 A decade of pushing the boundaries in proteomics, systems biology, and data-driven discovery. 🔬 Thank you to our collaborators, alumni & supporters!

Metabolic networks support cancer cell survival, proliferation, and malignant progression. Reactive oxygen species can damage macromolecules and induce pro-survival signaling pathways. Metabolites can modify chromatin and thus influence transcription.

Progress may be limited by our perception of what's possible. ⬛️ I share an example to encourage rethinking possibilities. Broader context: youtu.be/8nhjKSTqeOs?si…

Great to see the progress of these ideas & technologies 🚀

Protein abundance is regulated by many mechanisms. We quantified them across the single cells in a mammalian tissue: 1⃣ The results revealed simple rules ! 2⃣ They may alter how you interpret single-cell DNA(RNA)-sed data. ⚠️ See Andrew Leduc's 🧵 biorxiv.org/content/10.110…

Protein clearance rates vary across proteins in inverse proportion to the average cell-type doubling rate. This explains the degree to which protein clearance (degradation) sets protein abundance. biorxiv.org/content/10.110…

This work is the culmination of everything I have worked on since starting my PhD. 🧵

Multiplexed DIA enabled multimodal proteomics of ~ 10³ single cells, including quant of protein synthesis & degradation rates. Our technologies can increase the multiplexing along different dimensions (mass, time) and thus scale up throughput further. 🔽

I take pride in our research, but I am most proud of our team. Helping Andrew grow into a brilliant researcher has been a delight.

The regulation of nuclear-encoded mitochondrial proteins is fascinating. They are synthesized in a fully eukaryotic manner, with introns, splicing, and cytosolic ribosomes. Yet, their degradation is handed off to proteases that look and act bacterial. blog.slavovlab.net/2025/09/23/mix…

mRNA-mRNA correlations across the single cells from a tissue are rarely interpreted. They differ from the corresponding protein-protein correlations? 𝐖𝐡𝐲 ❓ biorxiv.org/content/10.110… 🧵

Proteasomes also play a major role in Alzheimer’s disease (AD). ⬛️ 20S proteasome abundance strongly declines in AD patients. => This decline parallels a reduction in 20S substrate proteins and leads to abnormal protein accumulation in AD. 👇 biorxiv.org/content/10.110…

What was once a projection is now reality. Exciting times for quantitative single-cell analysis !