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We recently shared our biorxiv preprint defining autophagy cargo. With this story Ian Smith created 'CARGO' a web interface to explore all of the data! See if your favorite proteins or organelles are degraded by autophagy during nutrient stress here: harperlab.connect.hms.harvard.edu/CARGO_Cellular…

Casanova is the new state of the art in de novo peptide sequencing. biorxiv.org/content/10.110…

Are you interested in studying phosphotyrosine modifications on proteins and want to save time and money? Check out our (Judit Villen) automated approach to enrich phosphotyrosine peptides using a pY superbinder or antibodies coupled to magnetic beads! biorxiv.org/content/10.110…

We are delighted to share our work, now available in nature: nature.com/articles/s4158… Our scoring system can be accessed here: kinase-library.phosphosite.org Coming soon --> the tyrosine kinome and an improved system with many new functionalities & features! #TheKinaseLibrary #KL

Excited to share our newest #preprint: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation. A great project with the Judit Villen Maitreya Dunham Matt Kaeberlein teams! 📄Read: biorxiv.org/content/10.110… 💾Explore: rlsproteomics.gs.washington.edu 1/9

Excited to share our preprint describing sci-Plex-GxE. A highly multiplexed workflow for combined exposure and genetic molecular screens at single-cell resolution. biorxiv.org/content/10.110…

We are very happy to announce that our latest story was published in Science Magazine yesterday! 🥳🎉 science.org/doi/10.1126/sc… (1/6)

Would you like to help us in our quest to map the human interactome via affinity-purification mass spectrometry? Become a key member of our team and help us identify interacting partners for every human protein! Please Retweet! linkedin.com/jobs/view/3547…

LOV-Turbo is published today in Nature Methods and plasmids for LOV-Turbo are available on Addgene, here: addgene.org/browse/article… We hope many people will use LOV-Turbo in exciting new ways! nature.com/articles/s4159… If you plan to use NanoLuc BRET to activate LOV-Turbo, please

Excited to attend #ASMS2023 and present our approach to study cellular aging using multidimensional proteomics:

Our preprint is now online! We define how the ER proteome is remodeled via autophagy during differentiation to neurons-- with combinatorial yet selective contributions of ER-phagy receptors. With @jwadeharperlab Ian Smith Julia Paoli Y. Jiang, J.Paulo. biorxiv.org/content/10.110…

Macroautophagy prioritizes turnover of ER and Golgi proteins during nutrient stress. Check out our latest work defining this and the discovery of a selective Golgi degradation pathway. Sharan Swarup, Ian Smith, @jwadeharperlab, @HarvardCellBio nature.com/articles/s4158…

Very happy to share our extensive and systematic Phosphoproteomic Stress Signaling Atlas that is now published NatureStructMolBiol: rdcu.be/doHAL Thanks to the Judit Villen lab and co-autors Anthony Barente , Noelle Fukuda & Ricard RodriguezMias! #proteomics #signaling #TeamMassSpec /1

Thrilled to announce the opening of the Srivatsan Lab (srivatsan-lab.com) at the Fred Hutch Cancer Center. The lab will be building new sequencing technologies to understand how our cells and bodies form over the course of development.

Congratulations Amanda Wiggenhorn, thesis work now in press Nature Communications. Inspired by the structure of the hypothalamic hormone TRH (pyroglu-his-pro-amide), Amanda shows “capping” defines a chemical motif present in many previously unknown bioactive peptides nature.com/articles/s4146…

So stoked to be sharing this! New proteomics map of human subcellular architecture. CRISPR + organelle IP mass spec at scale. Check out pre-print biorxiv.org/content/10.110… and organelles.czbiohub.org. 🙌 to fantastic team Chan Zuckerberg Biohub Network SF & collabs! 1/2

We have completed our thorough analysis of ER remodeling during neurogenesis by ER-phagy 🥳@jwadeharperlab Cristina Capitanio Ian Smith Julia Paoli @annabieber_ab Schulman lab @WilflingFlorian. And thanks to the reviewers for their work and insight. nature.com/articles/s4155…

How to pick a problem. Today in Cell. 1/53 cell.com/cell/fulltext/…

I am excited to share my first project in @jwadeharperlab for feedback. We used spatiotemporal proteomics (1) to describe how cells respond to NLRP3 agonists and (2) to profile the proximal proteome as NLRP3 trafficks to the site of inflammasome nucleation harperlab.pubpub.org/pub/nlrp3/

Comet's open-source search engine has been a boon for our proteomics methods developments (like real-time search). Over the last two-years we became curious whether Comet's search speeds could be improved to keep pace with modern instruments and large sample cohorts.